-transparent.png){kind=logo}
The present paper describes a study on rosette formation by human lymphoid cells with monkey erythrocytes (MRBC) obtained from two species: African green monkey (Cercopithecus aethiops) and rhesus monkey (Macaca mulata). All the lymphocyte preparations obtained from peripheral blood, tonsils, thymocytes, bone-marrow and spleen biopsies formed rosettes in variable proportions with erythrocytes obtained from these two monkey species tested. Cells of established lymphoblastoid cell lines characterized as being of either B- or T-cell origin also formed rosettes with MRBC. Taken together, the data obtained suggest that the MRBC-rosette assay cannot be reliably used for identifying lymphocyte subpopulations presently known to us, thus largely contradicting the observations previously reported by others on the use of this assay. In addition, we found that the binding sites for MRBC on the above lymphoid cells are highly sensitive to trypsin treatment and can fully be regenerated within 14-15 h when these treated cells are cultured at 37 degrees.
P C Patel, and
J Menezes, and
A Bourkas, and
G Dorval, and
G E Rivard, and
G Boulay, and
P Stanley, and
J H Joncas
October 1975,
Journal of immunology (Baltimore, Md. : 1950),
P C Patel, and
J Menezes, and
A Bourkas, and
G Dorval, and
G E Rivard, and
G Boulay, and
P Stanley, and
J H Joncas
January 1976,
Journal of immunological methods,
P C Patel, and
J Menezes, and
A Bourkas, and
G Dorval, and
G E Rivard, and
G Boulay, and
P Stanley, and
J H Joncas
April 1991,
The American journal of tropical medicine and hygiene,
P C Patel, and
J Menezes, and
A Bourkas, and
G Dorval, and
G E Rivard, and
G Boulay, and
P Stanley, and
J H Joncas
January 1977,
Immunological communications,
P C Patel, and
J Menezes, and
A Bourkas, and
G Dorval, and
G E Rivard, and
G Boulay, and
P Stanley, and
J H Joncas
January 1978,
International archives of allergy and applied immunology,
P C Patel, and
J Menezes, and
A Bourkas, and
G Dorval, and
G E Rivard, and
G Boulay, and
P Stanley, and
J H Joncas
July 1974,
European journal of immunology,
P C Patel, and
J Menezes, and
A Bourkas, and
G Dorval, and
G E Rivard, and
G Boulay, and
P Stanley, and
J H Joncas
April 1984,
Orvosi hetilap,
P C Patel, and
J Menezes, and
A Bourkas, and
G Dorval, and
G E Rivard, and
G Boulay, and
P Stanley, and
J H Joncas
February 1977,
Zeitschrift fur Immunitatsforschung. Immunobiology,
P C Patel, and
J Menezes, and
A Bourkas, and
G Dorval, and
G E Rivard, and
G Boulay, and
P Stanley, and
J H Joncas
January 2000,
Haematologia,
P C Patel, and
J Menezes, and
A Bourkas, and
G Dorval, and
G E Rivard, and
G Boulay, and
P Stanley, and
J H Joncas
March 1980,
Zhonghua nei ke za zhi,
