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We offer an assessment of the accuracy of the thin-layer agarose fluorescent technique of Elevitch et al. [Am. J. Clin. Pathol. 46, 692 (1966)]. We used semi-purified human lactate dehydrogenase isoenzymes 1 and 5. Both the lactate lead to pyruvate and pyruvate lead to lactate assays [Clin. Chem. 20, 1462 (1974)] appear to give, within the errors of the techniques used, a substantially unbiased estimate of both LD-1 and LD-5, although this must remain a provisional conclusion until a definitive method of assay for the total and isoenzymic LD activities is created. Introducing a buffer into the substrate mixture (lactate lead to pyruvate assay) had no effect on these findings except at extremes of pH, when marked inaccuracies occurred.
D McKenzie, and
P I Clark, and
A R Henderson
December 1983,
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D McKenzie, and
P I Clark, and
A R Henderson
October 1981,
Clinical chemistry,
D McKenzie, and
P I Clark, and
A R Henderson
February 1979,
Clinical chemistry,
D McKenzie, and
P I Clark, and
A R Henderson
September 1981,
Clinical chemistry,
D McKenzie, and
P I Clark, and
A R Henderson
October 1974,
Rinsho byori. The Japanese journal of clinical pathology,
D McKenzie, and
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A R Henderson
October 1996,
Clinical chemistry,
D McKenzie, and
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A R Henderson
November 1989,
Clinical chemistry,
D McKenzie, and
P I Clark, and
A R Henderson
January 1980,
Laboratornoe delo,
D McKenzie, and
P I Clark, and
A R Henderson
April 1986,
Clinical chemistry,
D McKenzie, and
P I Clark, and
A R Henderson
January 1979,
Clinical chemistry,
