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We describe an automated enzymatic reaction-rate method for spectrophotometric determination of lactate in serum with a miniature centrifugal analyzer. The L(+) -lactate is selectively oxidized in the presence of lactate dehydrogenase (EC 1.1.1.27) and NAD+ to form NADH, which is measured from its absorption. Reaction rates are determined automatically, and unknown concentrations are calculated from a computer-generated calibration curve with aqueous lithium lactate standards. Lactate concentrations in the range 0.32-1.6 mug/4 mul (80-400 mg/liter) of sample were determined with relative errors and coefficient of variation of 4.8%. Analytical recovery of lactate added to pooled serum was 89-112% (average, 101%). Comparison with a kit ("Rapid Lactate") method gave a correlation coefficient squared of 0.979 over a concentration range of 39-779 mg/liter.
T P Hadjiioannou, and
S I Hadjiioannou, and
S D Brunk, and
H V Malmstadt
September 1974,
Clinical chemistry,
T P Hadjiioannou, and
S I Hadjiioannou, and
S D Brunk, and
H V Malmstadt
October 1974,
Clinical chemistry,
T P Hadjiioannou, and
S I Hadjiioannou, and
S D Brunk, and
H V Malmstadt
March 1982,
Clinical chemistry,
T P Hadjiioannou, and
S I Hadjiioannou, and
S D Brunk, and
H V Malmstadt
August 1976,
Clinical chemistry,
T P Hadjiioannou, and
S I Hadjiioannou, and
S D Brunk, and
H V Malmstadt
January 1979,
Annals of clinical and laboratory science,
T P Hadjiioannou, and
S I Hadjiioannou, and
S D Brunk, and
H V Malmstadt
January 1981,
Annales de biologie clinique,
T P Hadjiioannou, and
S I Hadjiioannou, and
S D Brunk, and
H V Malmstadt
August 1975,
Clinical chemistry,
T P Hadjiioannou, and
S I Hadjiioannou, and
S D Brunk, and
H V Malmstadt
March 1974,
Clinical chemistry,
T P Hadjiioannou, and
S I Hadjiioannou, and
S D Brunk, and
H V Malmstadt
February 1975,
Clinical chemistry,
T P Hadjiioannou, and
S I Hadjiioannou, and
S D Brunk, and
H V Malmstadt
December 1976,
Clinical chemistry,
