BIOMAT Database Logo BIOMAT Database Logo

Use of polyclonal antibodies against carcinogen-DNA adducts in analysis of carcinogenesis. 1998

T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
First Department of Pathology, Nagoya City University Medical School, Nagoya, Japan. tshirai@med.nagoya-cu.ac.jp

Polyclonal antibodies against 3,2'-dimethyl-4-aminobiphenyl (DMAB)- and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-DNA adducts were raised for their immunohistochemical demonstration in paraffin-embedded sections. Both carcinogens target multiorgans in rats with colon, mammary glands and prostate as common tumor sites. PhIP is particularly important because it is a cooked food-derived carcinogen to which man is exposed on a daily bases. Dose-related nuclear staining in various tissue of rats was observed 24 h after single applications of both carcinogens but no observable decrease in staining intensity was evident in most organs by 168 h. Staining specificity in terms of sites of tumor development was lacking. However, in target organs, semiquantitative data on adduct formation correlated with alteration in tumor response by modifying factor(s). Furthermore, human prostate tissue implanted into nude mice showed positive staining. Thus, the antibodies can be applied as tools to clarify tissue or cell-specific carcinogenesis, carcinogen-exposure levels and metabolic activation in various species, including man after tissue transplantation.

UI MeSH Term Description Entries
D007093 Imidazoles Compounds containing 1,3-diazole, a five membered aromatic ring containing two nitrogen atoms separated by one of the carbons. Chemically reduced ones include IMIDAZOLINES and IMIDAZOLIDINES. Distinguish from 1,2-diazole (PYRAZOLES).
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008297 Male Males
D011467 Prostate A gland in males that surrounds the neck of the URINARY BLADDER and the URETHRA. It secretes a substance that liquefies coagulated semen. It is situated in the pelvic cavity behind the lower part of the PUBIC SYMPHYSIS, above the deep layer of the triangular ligament, and rests upon the RECTUM. Prostates
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002273 Carcinogens Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included. Carcinogen,Oncogen,Oncogens,Tumor Initiator,Tumor Initiators,Tumor Promoter,Tumor Promoters,Initiator, Tumor,Initiators, Tumor,Promoter, Tumor,Promoters, Tumor
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000611 Aminobiphenyl Compounds Biphenyl compounds substituted in any position by one or more amino groups. Permitted are any substituents except fused rings. Biphenylamines,Compounds, Aminobiphenyl
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
Immunoaffinity chromatography of carcinogen DNA adducts with polyclonal antibodies directed against benzo[a]pyrene diol-epoxide-DNA.
July 1986, Journal of the National Cancer Institute,
T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
Antibodies to carcinogen-DNA adducts.
September 1981, Journal of the National Cancer Institute,
T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
Quantitation of carcinogen-DNA adducts with monoclonal antibodies.
January 1987, Progress in experimental tumor research,
T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
Use of monoclonal and polyclonal antibodies against DNA adducts for the detection of DNA lesions in isolated DNA and in single cells.
October 1985, Environmental health perspectives,
T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
The use of antibodies to detect carcinogen - DNA adducts in vivo and in vitro.
January 1983, Progress in clinical and biological research,
T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
The use of rabbit polyclonal antibodies for the isolation of carcinogen-adducted DNA by immunoprecipitation.
January 1991, Molecular carcinogenesis,
T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
Detection of carcinogen-DNA adducts in human cells and antibodies to these adducts in human sera.
January 1987, Progress in experimental tumor research,
T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
Biochemical (postlabelling) methods for analysis of carcinogen-DNA adducts.
January 1984, IARC scientific publications,
T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
Monoclonal and polyclonal antibodies against acetaldehyde-containing epitopes in acetaldehyde-protein adducts.
October 1986, Proceedings of the National Academy of Sciences of the United States of America,
T Shirai, and S Takahashi, and L Cui, and Y Yamada, and M Tada, and F F Kadlubar, and N Ito
Carcinogen-DNA and carcinogen-protein adducts in molecular epidemiology.
January 1997, IARC scientific publications,
Copied contents to your clipboard!