Biomaterial Database

[cDNA cloning of human testicular follicle-stimulating hormone receptor and analysis of its in vitro function]. 1998

T Aihara

Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.

A cDNA was cloned from human testis cDNA library by the use of rat FSH receptor cDNA as a probe. Transformation of 293 cells with the cloned cDNA led to expression of specific human FSH binding sites with high affinity (Kd: 1.5 x 10(-9) M), ligand dose-dependent production of cAMP and promotion of phosphatidyl inositol turnover, indicating that the cloned cDNA must encode a human FSH receptor. Northern blot analysis using human ovarian tissues revealed a high expression of mRNA at the stage of early follicular phase, but not luteal phase. Cross-linking of ligand with the expressed human FSH receptor showed that the molecular mass of the receptor should be 76 kDa, consistent with the estimated size of deduced amino acid sequence from the cloned cDNA. A polymorphism was found at 680th amino acid Ser or Asn, in the C-terminal region of the receptor although the influence of this difference upon the receptor function was not determined obviously in this study yet. The incidence of the polymorphism in the C-terminal region was not significantly correlated to the reproductive failures in 70 female cases of endometriosis, hyperprolactinemia, amenorrhea, PCOD or POF. However, elucidation of the structure and function in human FSH receptor using the cDNA will be applicable to diagnosis of some clinical cases associated with abnormal FSH receptor gene in the future.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011110	Polymorphism, Genetic	The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.	Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D011962	Receptors, FSH	Cell surface proteins that bind FOLLICLE STIMULATING HORMONE with high affinity and trigger intracellular changes influencing the behavior of cells.	FSH Receptors,Follicle-Stimulating Hormone Receptors,Receptors, Follicle-Stimulating Hormone,FSH Receptor,Follicle-Stimulating Hormone Receptor,Follicle Stimulating Hormone Receptor,Follicle Stimulating Hormone Receptors,Hormone Receptor, Follicle-Stimulating,Hormone Receptors, Follicle-Stimulating,Receptor, FSH,Receptor, Follicle-Stimulating Hormone,Receptors, Follicle Stimulating Hormone
D012150	Polymorphism, Restriction Fragment Length	Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.	RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia