The DNA-binding drugs mithramycin and chromomycin are powerful inducers of erythroid differentiation of human K562 cells. 1999

N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
Department of Biochemistry and Molecular Biology, University of Ferrara, Italy.

The human leukaemic K562 cell line can be induced in vitro to undergo erythroid differentiation by a variety of chemical compounds, including haemin, butyric acid, 5-azacytidine and cytosine arabinoside. Differentiation of K562 cells is associated with an increased expression of embryo-fetal globin genes, such as the zeta, epsilon and gamma globin genes. Therefore the K562 cell line has been proposed as a useful in vitro model system to determine the therapeutic potential of new differentiating compounds as well as to study the molecular mechanism(s) regulating changes in the expression of embryonic and fetal human globin genes. Inducers of erythroid differentiation which stimulate gamma-globin synthesis could be considered for possible use in the experimental therapy of those haematological diseases associated with a failure in the expression of adult beta-globin genes. In this paper we demonstrated that the G + C selective DNA-binding drugs chromomycin and mithramycin were powerful inducers of erythroid differentiation of K562 cells. Erythroid differentiation was associated with an increase in the accumulation of (a) Hb Gower 1 and Hb Portland and (b) gamma-globin mRNA.

UI MeSH Term Description Entries
D008926 Plicamycin A tricyclic pentaglycosidic antibiotic from Streptomyces strains that inhibits RNA and protein synthesis by adhering to DNA. It is used as a fluorescent dye and as an antineoplastic agent, especially in bone and testicular tumors. Plicamycin is also used to reduce hypercalcemia, especially that due to malignancies. Aureolic Acid,Mithramycin,Mitramycin
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002865 Chromomycins A complex of several closely related glycosidic antibiotics from Streptomyces griseus. The major component, CHROMOMYCIN A3, is used as a fluorescent stain of DNA where it attaches and inhibits RNA synthesis. It is also used as an antineoplastic agent, especially for solid tumors. Chromomycin
D003850 Deoxyribonuclease I An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA. DNase I,Streptodornase,DNA Endonuclease,DNA Nicking Enzyme,DNAase I,Dornavac,Endonuclease I,Nickase,Pancreatic DNase,T4-Endonuclease II,T7-Endonuclease I,Thymonuclease,DNase, Pancreatic,Endonuclease, DNA,T4 Endonuclease II,T7 Endonuclease I
D005914 Globins A superfamily of proteins containing the globin fold which is composed of 6-8 alpha helices arranged in a characterstic HEME enclosing structure. Globin
D006454 Hemoglobins The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements. Eryhem,Ferrous Hemoglobin,Hemoglobin,Hemoglobin, Ferrous
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D015152 Blotting, Northern Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. Northern Blotting,Blot, Northern,Northern Blot,Blots, Northern,Blottings, Northern,Northern Blots,Northern Blottings

Related Publications

N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
January 1981, Experimental hematology,
N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
March 1999, European journal of biochemistry,
N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
January 1983, Leukemia research,
N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
December 2010, Biochemistry,
N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
June 2014, Oncotarget,
N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
March 2001, Annals of neurology,
N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
November 2012, Toxicology and applied pharmacology,
N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
July 2000, Biochemical pharmacology,
N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
May 2011, Molecular nutrition & food research,
N Bianchi, and F Osti, and C Rutigliano, and F G Corradini, and E Borsetti, and M Tomassetti, and C Mischiati, and G Feriotto, and R Gambari
January 1991, Leukemia research,
Copied contents to your clipboard!