Biomaterial Database

Inhibition of passive hemagglutination- a sensitive test for host antigen detection and evaluation of virus purity. 1976

G R Ghyka, and A Pascaru

Inhibition of passive hemagglutination (IPHA) proved to be a very sensitive and specific reaction for immunochemical detection and dosage of host antigens or proteins impurifying various virus suspensions. Thus, IPHA is able to detect bovine serum albumin amounts of 0.05 mug/ml, and chorio-allantoic membrane antigens at a concentration of 0.35 mug/ml. The estimation of the ratio between specific activity (hemagglutinating (HA) titer in the case of influenza A0PR8 virus) and the amount of host proteins (evaluated by IPHA) is recommended as a criterion of virus purity. In the case of highly purified virus suspensions this criterion is more reliable than the presently used ratio between HA titer and total protein amount (estimated by Lowry's reaction).

UI	MeSH Term	Description	Entries
D009975	Orthomyxoviridae	A family of RNA viruses causing INFLUENZA and other respiratory diseases. Orthomyxoviridae includes INFLUENZAVIRUS A; INFLUENZAVIRUS B; INFLUENZAVIRUS C; INFLUENZAVIRUS D; ISAVIRUS; and THOGOTOVIRUS.	Influenza Viruses,Myxoviruses,Orthomyxoviruses,Influenza Virus,Myxovirus,Orthomyxovirus
D006385	Hemagglutination Inhibition Tests	Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.	Hemagglutination Inhibition Test,Inhibition Test, Hemagglutination,Inhibition Tests, Hemagglutination,Test, Hemagglutination Inhibition,Tests, Hemagglutination Inhibition
D000956	Antigens, Viral	Substances elaborated by viruses that have antigenic activity.	Viral Antigen,Viral Antigens,Antigen, Viral
D014764	Viral Proteins	Proteins found in any species of virus.	Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral