Biomaterial Database

[Expression and detection of integrin alpha 5 beta 1, fibronectin and its fragment in patients with hepatocellular carcinoma]. 1997

J Zhao, and W Zhai, and Y Zhang

Department of Pathology, Shanghai Medical University.

OBJECTIVE To study the relationship between the expression of integrin alpha 5 beta 1, fibronectin (FN) and its fragment (malignant disease-associated DNA-binding protein 2 MAD2) and the biologic behavior of human hepatocellular carcinoma HCC) as well as the significance of MAD2 detection in plasma. METHODS Integrin alpha 5 beta 1, FN and MAD2 were localized by immunohistochemical methods and quantified by image analysis in tumor and peripheral liver tissues from 40 HCC cases. The concentration of FN and MAD2 in the plasma samples from 30/40 cases were examined using the ELISA method. RESULTS The expression of integrin alpha 5 beta 1, FN and MAD2 in well differentiated HCC were not significantly different from that of normal tissues, while they were markedly decreased or undetectable in mode rately and poorly differentiated HCC. Decreased expression of integrin alpha 5 beta 1 and FN at sites where HCC invaded the connective tissue and enhanced expression on the HCC cells around cancerous emboli were also noted. The mean concentration of plasma MAD2 in patients with HCC were significantly increased when compared to those with chronic liver disease and normal subjects. CONCLUSIONS The changes in integrin alpha 5 beta 1 and FN expression may be associated with HCC differentiation, invasion and metastasis. The detection of plasma MAD2 in patients with HCC may have clinical significance.

UI	MeSH Term	Description	Entries
D008113	Liver Neoplasms	Tumors or cancer of the LIVER.	Cancer of Liver,Hepatic Cancer,Liver Cancer,Cancer of the Liver,Cancer, Hepatocellular,Hepatic Neoplasms,Hepatocellular Cancer,Neoplasms, Hepatic,Neoplasms, Liver,Cancer, Hepatic,Cancer, Liver,Cancers, Hepatic,Cancers, Hepatocellular,Cancers, Liver,Hepatic Cancers,Hepatic Neoplasm,Hepatocellular Cancers,Liver Cancers,Liver Neoplasm,Neoplasm, Hepatic,Neoplasm, Liver
D010446	Peptide Fragments	Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.	Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D005353	Fibronectins	Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.	Cold-Insoluble Globulins,LETS Proteins,Fibronectin,Opsonic Glycoprotein,Opsonic alpha(2)SB Glycoprotein,alpha 2-Surface Binding Glycoprotein,Cold Insoluble Globulins,Globulins, Cold-Insoluble,Glycoprotein, Opsonic,Proteins, LETS,alpha 2 Surface Binding Glycoprotein
D006528	Carcinoma, Hepatocellular	A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.	Hepatocellular Carcinoma,Hepatoma,Liver Cancer, Adult,Liver Cell Carcinoma,Liver Cell Carcinoma, Adult,Adult Liver Cancer,Adult Liver Cancers,Cancer, Adult Liver,Cancers, Adult Liver,Carcinoma, Liver Cell,Carcinomas, Hepatocellular,Carcinomas, Liver Cell,Cell Carcinoma, Liver,Cell Carcinomas, Liver,Hepatocellular Carcinomas,Hepatomas,Liver Cancers, Adult,Liver Cell Carcinomas
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015703	Antigens, CD	Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.	CD Antigen,Cluster of Differentiation Antigen,Cluster of Differentiation Marker,Differentiation Antigens, Leukocyte, Human,Leukocyte Differentiation Antigens, Human,Cluster of Differentiation Antigens,Cluster of Differentiation Markers,Antigen Cluster, Differentiation,Antigen, CD,CD Antigens,Differentiation Antigen Cluster,Differentiation Marker Cluster,Marker Cluster, Differentiation
D017469	Receptors, Fibronectin	Specific cell surface receptors which bind to FIBRONECTINS. Studies have shown that these receptors function in certain types of adhesive contact as well as playing a major role in matrix assembly. These receptors include the traditional fibronectin receptor, also called INTEGRIN ALPHA5BETA1 and several other integrins.	Fibronectin Receptors
D039482	Integrin alpha5	This integrin alpha subunit combines with INTEGRIN BETA1 to form a receptor (INTEGRIN ALPHA5BETA1) that binds FIBRONECTIN and LAMININ. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds.	Antigens, CD49e,CD49e Antigens,CD49e Antigen,Integrin alphaF,Platelet Glycoprotein Ic,VLA-5 alpha Chain,alpha(5) Integrin,alpha5 Integrin,Antigen, CD49e,Glycoprotein Ic, Platelet,Ic, Platelet Glycoprotein,Integrin, alpha5,VLA 5 alpha Chain,alpha Chain, VLA-5,alpha5, Integrin,alphaF, Integrin