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OBJECTIVE To study the intracellular free calcium ([Ca2+]i) in normal and hypertrophic left ventricular myocytes isolated from adult rat hearts and the effects of praeruptorine C (Pra-C) on them. METHODS [Ca2+]i of single myocyte was measured with Fura 2-AM. RESULTS The resting [Ca2+]i was 87 +/- 4 nmol.L-1 in normal left ventricular myocytes, 123 +/- 7 nmol.L-1 in hypertrophied myocytes. After exposure to KCl (20, 40, and 60 mmol.L-1), the [Ca2+]i were increased by 66%, 141%, and 268% in normal myocytes, and 77%, 185%, and 243% in hypertrophic myocytes, respectively. Pra-C (1, 10, and 100 mumol.L-1) concentration-dependently inhibited the [Ca2+]i elevation caused by KCl (35 mmol.L-1) or norepinephrine (20 mumol.L-1) in both normal and hypertrophied myocytes. All of the effects of Pra-C were similar to that of nifedipine. CONCLUSIONS [Ca2+]i of hypertrophied myocytes was higher than that of normal ones and Pra-C decrease the [Ca2+]i elevation in left ventricular myocytes resulted from its calcium channel blockade.
L Sun, and
M R Rao, and
J X Wang
June 1995,
The Journal of clinical investigation,
L Sun, and
M R Rao, and
J X Wang
December 1988,
The American journal of physiology,
L Sun, and
M R Rao, and
J X Wang
October 2005,
Sheng li xue bao : [Acta physiologica Sinica],
L Sun, and
M R Rao, and
J X Wang
December 2002,
Sheng li xue bao : [Acta physiologica Sinica],
L Sun, and
M R Rao, and
J X Wang
April 2004,
Sheng li xue bao : [Acta physiologica Sinica],
L Sun, and
M R Rao, and
J X Wang
April 2007,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
L Sun, and
M R Rao, and
J X Wang
February 2006,
Zhonghua xin xue guan bing za zhi,
L Sun, and
M R Rao, and
J X Wang
January 1992,
Life sciences,
L Sun, and
M R Rao, and
J X Wang
October 1998,
Pflugers Archiv : European journal of physiology,
L Sun, and
M R Rao, and
J X Wang
September 2000,
Circulation research,
