BIOMAT Database Logo BIOMAT Database Logo

Vaccination of mice with DNA plasmids coding for the Chlamydia trachomatis major outer membrane protein elicits an immune response but fails to protect against a genital challenge. 1999

S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
Department of Pathology, Medical Sciences I, University of California, Irvine 92697-4800, USA.

A DNA plasmid encoding the gene of the major outer membrane protein (MOMP) of the Chlamydia trachomatis mouse pneumonitis (MoPn) serovar and three plasmids containing the variable domains (VD) of the MOMP were constructed. Female mice were inoculated with the plasmids and 60 days later were challenged in the genital tract with C. trachomatis. Six weeks after challenge female mice were caged with male mice and the course of the mating followed. Mice immunized with the MOMP plasmids mounted weak humoral and cell mediated immune responses. However, following the genital challenge no significant differences in vaginal shedding were observed between the groups immunized with the MOMP and control plasmids. In addition, the fertility rates were similar in the experimental and negative control groups. In conclusion, vaccination with DNA plasmids encoding the MOMP elicited a modest immune response but did not protect against infection or disease.

UI MeSH Term Description Entries
D008297 Male Males
D008815 Mice, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation. Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D002690 Chlamydia Infections Infections with bacteria of the genus CHLAMYDIA. Infections, Chlamydia,Chlamydia Infection,Infection, Chlamydia
D002692 Chlamydia trachomatis Type species of CHLAMYDIA causing a variety of ocular and urogenital diseases.
D005260 Female Females
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D005831 Genital Diseases, Female Pathological processes involving the female reproductive tract (GENITALIA, FEMALE). Gynecologic Diseases,Female Genital Diseases,Diseases, Female Genital,Diseases, Gynecologic,Female Genital Disease,Genital Disease, Female,Gynecologic Disease
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
Vaccination with major outer membrane protein proteosomes elicits protection in mice against a Chlamydia respiratory challenge.
November 2013, Microbes and infection,
S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
Immunization with the Chlamydia trachomatis mouse pneumonitis major outer membrane protein can elicit a protective immune response against a genital challenge.
October 2001, Infection and immunity,
S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
Vaccination with the recombinant major outer membrane protein elicits long-term protection in mice against vaginal shedding and infertility following a Chlamydia muridarum genital challenge.
January 2020, NPJ vaccines,
S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
Vaccination with the recombinant major outer membrane protein elicits antibodies to the constant domains and induces cross-serovar protection against intranasal challenge with Chlamydia trachomatis.
May 2013, Infection and immunity,
S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
Monoclonal immunoglobulin A antibody to the major outer membrane protein of the Chlamydia trachomatis mouse pneumonitis biovar protects mice against a chlamydial genital challenge.
April 1997, Vaccine,
S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
Immune response to the Chlamydia trachomatis outer membrane protein PorB.
October 2004, Vaccine,
S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
Immunization with the Chlamydia trachomatis major outer membrane protein, using the outer surface protein A of Borrelia burgdorferi as an adjuvant, can induce protection against a chlamydial genital challenge.
March 2003, Vaccine,
S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
Induction of protective immunity against Chlamydia trachomatis genital infection by a vaccine based on major outer membrane protein-lipophilic immune response-stimulating complexes.
December 2000, Infection and immunity,
S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
DNA vaccination with the major outer-membrane protein gene induces acquired immunity to Chlamydia trachomatis (mouse pneumonitis) infection.
October 1997, The Journal of infectious diseases,
S Pal, and K M Barnhart, and Q Wei, and A M Abai, and E M Peterson, and L M de la Maza
Immunoassay for detecting Chlamydia trachomatis major outer membrane protein.
September 1983, Journal of clinical microbiology,
Copied contents to your clipboard!