Biomaterial Database

[Effects of hypoxia on the morphology and proliferation of cultured pulmonary arterial smooth muscle cell]. 1997

J Liu, and D Luo, and P Wang

Department of Pathophysiology, Third Military Medical College, Chongqing.

The effects of hypoxia (2.5% O2 and/or < 1% O2) on bovine pulmonary arterial smooth muscle cell (PASM) proliferation and phenotype change were investigated using immunocytochemical analysis and H- TdR incorporation. The results showed that hypoxia initiated the change of PASM from contractile phenotype to synthetic phenotype. The synthetic phenotype smooth muscle cell was characterized by increase in endoplasmic reticulum and mitochondria, and by decrease in alpha-actin and muscle fiber at the end of 24 h hypoxia. Then the endoplasmic reticulum became dilated, the mitochondria became swollen and myelin figure appeared after 48 h hypoxic exposure. PASM 3H-TdR incorporation was decreased at the end of 6 h hypoxia (P < 0.05), then increased gradually at the end of 12 h. PASM DNA synthesis was significantly stimulated by 24 h hypoxia (P < 0.05). Flow cytometric DNA analysis revealed that hypoxia induced significant enhancement of G2/M phase of PASMs, decreased G0/G1 phase of PASMs (P < 0.001), and only increased S phase of PASMs in 12 h anoxia group (P < 0.05), but decreased S phase of PASMs from 24 h to 48 h (P < 0.001). Immunocytochemical reaction of proliferating cell nuclear antigen (PCNA) in PASM under anoxic condition was more stronger than that of normoxic PASM at 24 h. These results suggest that hypoxia may at first inhibit and then stimulate PASM proliferation and induce phenotype change, which may lead to the development of hypoxic pulmonary hypertension.

UI	MeSH Term	Description	Entries
D009131	Muscle, Smooth, Vascular	The nonstriated involuntary muscle tissue of blood vessels.	Vascular Smooth Muscle,Muscle, Vascular Smooth,Muscles, Vascular Smooth,Smooth Muscle, Vascular,Smooth Muscles, Vascular,Vascular Smooth Muscles
D011651	Pulmonary Artery	The short wide vessel arising from the conus arteriosus of the right ventricle and conveying unaerated blood to the lungs.	Arteries, Pulmonary,Artery, Pulmonary,Pulmonary Arteries
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002453	Cell Cycle	The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.	Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004721	Endoplasmic Reticulum	A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)	Ergastoplasm,Reticulum, Endoplasmic
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015687	Cell Hypoxia	A condition of decreased oxygen content at the cellular level.	Anoxia, Cellular,Cell Anoxia,Hypoxia, Cellular,Anoxia, Cell,Anoxias, Cell,Anoxias, Cellular,Cell Anoxias,Cell Hypoxias,Cellular Anoxia,Cellular Anoxias,Cellular Hypoxia,Cellular Hypoxias,Hypoxia, Cell,Hypoxias, Cell,Hypoxias, Cellular
D018809	Proliferating Cell Nuclear Antigen	Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.	Antigen, Proliferating Cell Nuclear,PCNA