Biomaterial Database

Determination of daminozide in apples and apple leaves by liquid chromatography--mass spectrometry. 1999

H G Mol, and R C van Dam, and R J Vreeken, and O M Steijger

TNO Nutrition and Food Research Institute, Department of Pesticides, Zeist, The Netherlands.

A straightforward and efficient method was developed for the determination of intact daminozide in apples and apple leaves. After extraction with methanol and a clean-up step using a graphitized carbon cartridge, the extract was analysed by ion-trap liquid chromatography-tandem mass spectrometry (LC-MS-MS) using atmospheric pressure chemical ionisation in the positive ion mode. Recoveries for apple were 98-102% with a R.S.D. < or = 11% (n = 6) and for leaves were 112-116% with a R.S.D. < or = 18% (n = 6). The limits of detection were 0.008 and 0.02 mg/kg for apples and leaves, respectively.

UI	MeSH Term	Description	Entries
D010937	Plant Growth Regulators	Any of the hormones produced naturally in plants and active in controlling growth and other functions. There are three primary classes: auxins, cytokinins, and gibberellins.	Phytohormone,Phytohormones,Plant Growth Regulator,Plant Hormone,Plant Hormones,Growth Regulators, Plant,Regulators, Plant Growth,Growth Regulator, Plant,Hormone, Plant,Hormones, Plant,Regulator, Plant Growth
D002853	Chromatography, Liquid	Chromatographic techniques in which the mobile phase is a liquid.	Liquid Chromatography
D005638	Fruit	The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds.	Berries,Legume Pod,Plant Aril,Plant Capsule,Aril, Plant,Arils, Plant,Berry,Capsule, Plant,Capsules, Plant,Fruits,Legume Pods,Plant Arils,Plant Capsules,Pod, Legume,Pods, Legume
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D013058	Mass Spectrometry	An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.	Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass
D013386	Succinates	Derivatives of SUCCINIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,4-carboxy terminated aliphatic structure.	Succinic Acids,Acids, Succinic