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Macrophage migration inhibitory factor (MIF) plays an important role in inflammation and immunity via autocrine/paracrine and endocrine routes. We examined the presence of MIF in the synovial fluids of rheumatoid arthritis (RA) patients. The content of MIF in the synovial fluid was quantitated by enzyme-linked immunosorbent assay which revealed that the concentration of MIF for RA patients was 85. 7+/-35.2 ng/ml (mean+/-SD) (n=25). In comparison, the concentrations for osteoarthritis patients and normal volunteers were 19.5+/-5.3 ng/ml (n=12) and 10.4+/-1.1 ng/ml (n=5), respectively. The expression of MIF mRNA and presence of MIF protein in the synovial tissues of RA were demonstrated by Northern blot and Western blot analyses, respectively. Immunohistochemical analysis revealed that positive staining was largely observed in the cytoplasm of infiltrating T lymphocytes, which might be the major source of MIF detected in the synovial fluids. The pathophysiological role of MIF in RA remains to be elucidated; however, the present results for the first time suggest the possibility that MIF is involved in the potentiation of inflammatory and immunological responses in rheumatoid joints.
S Onodera, and
H Tanji, and
K Suzuki, and
K Kaneda, and
Y Mizue, and
A Sagawa, and
J Nishihira
January 2000,
The Journal of biological chemistry,
S Onodera, and
H Tanji, and
K Suzuki, and
K Kaneda, and
Y Mizue, and
A Sagawa, and
J Nishihira
January 2005,
Frontiers in bioscience : a journal and virtual library,
S Onodera, and
H Tanji, and
K Suzuki, and
K Kaneda, and
Y Mizue, and
A Sagawa, and
J Nishihira
May 1977,
Arthritis and rheumatism,
S Onodera, and
H Tanji, and
K Suzuki, and
K Kaneda, and
Y Mizue, and
A Sagawa, and
J Nishihira
February 2010,
Cornea,
S Onodera, and
H Tanji, and
K Suzuki, and
K Kaneda, and
Y Mizue, and
A Sagawa, and
J Nishihira
May 2002,
Rheumatology (Oxford, England),
S Onodera, and
H Tanji, and
K Suzuki, and
K Kaneda, and
Y Mizue, and
A Sagawa, and
J Nishihira
January 1984,
Nihon Seikeigeka Gakkai zasshi,
S Onodera, and
H Tanji, and
K Suzuki, and
K Kaneda, and
Y Mizue, and
A Sagawa, and
J Nishihira
January 1998,
Archives of andrology,
S Onodera, and
H Tanji, and
K Suzuki, and
K Kaneda, and
Y Mizue, and
A Sagawa, and
J Nishihira
April 2007,
American journal of obstetrics and gynecology,
S Onodera, and
H Tanji, and
K Suzuki, and
K Kaneda, and
Y Mizue, and
A Sagawa, and
J Nishihira
May 2010,
Journal of cancer research and clinical oncology,
S Onodera, and
H Tanji, and
K Suzuki, and
K Kaneda, and
Y Mizue, and
A Sagawa, and
J Nishihira
January 2007,
Arthritis research & therapy,
