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Crystallization and preliminary X-ray diffraction of Trypanosoma cruzi dUTPase. 1999
V Bernier-Villamor, and
A Camacho, and
D González-Pacanowska, and
E Cedergren-Zeppezauer, and
A Antson, and
K S Wilson
Instituto de Parasitología y Biomedicina 'López Neyra', Ventanilla, 11, PO 18006 Granada, Spain. vbernier@ipb.csic.es
Crystals of Trypanosoma cruzi dUTPase have been grown. Two different morphologies are observed, depending on the molecular weight of the PEG used as precipitating agent in the mother liquor, both having a hexagonal unit cell with similar dimensions. Complete X-ray diffraction data have been collected to low resolution for one of the forms. The space group is P6322, with unit-cell dimensions a = 134.15, c = 147.05 A. Peaks in the self-rotation function and the solvent content are consistent with two molecules of dUTPase per asymmetric unit.
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