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OBJECTIVE To evaluate histologically and immunohistochemically long-term changes in rabbit cornea following excimer laser ablation. METHODS Excimer laser keratectomy was performed on 12 eyes of 6 rabbits. The corneas were subjected to histological and immunohistochemical studies 2 and 3 years after treatment. RESULTS In all specimens, epithelial basement membrane of the ablated central cornea showed undulating and spike-pattern invasion into the epithelial side. The collagen lamellae showed irregularities in the subepithelial stroma. Immunohistochemically, the subepithelial stroma showed positive reaction to anti-type III collagen antibody. Staining for anti-IV collagen antibody showed a linear pattern in the epithelial basement membrane and was positive in the subepithelial stroma of the ablated central cornea. These positive reactions to anti-type III and type IV collagen antibodies in the stroma showed the localized presence of scar tissue in the ablated stroma. Antibody to type VII collagen showed weak reaction in the basement membrane, suggesting incomplete regeneration of anchoring fibrils. CONCLUSIONS The healing process was still incomplete 3 years after excimer laser keratotomy in rabbits.
K Nakayasu, and
Y Watanabe, and
T Gotoh, and
T Kato, and
T Ishikawa, and
A Kanai
November 1990,
Archives of ophthalmology (Chicago, Ill. : 1960),
K Nakayasu, and
Y Watanabe, and
T Gotoh, and
T Kato, and
T Ishikawa, and
A Kanai
October 2004,
Journal of cataract and refractive surgery,
K Nakayasu, and
Y Watanabe, and
T Gotoh, and
T Kato, and
T Ishikawa, and
A Kanai
July 1996,
[Zhonghua yan ke za zhi] Chinese journal of ophthalmology,
K Nakayasu, and
Y Watanabe, and
T Gotoh, and
T Kato, and
T Ishikawa, and
A Kanai
January 2005,
Journal of refractive surgery (Thorofare, N.J. : 1995),
K Nakayasu, and
Y Watanabe, and
T Gotoh, and
T Kato, and
T Ishikawa, and
A Kanai
November 2004,
Archives of ophthalmology (Chicago, Ill. : 1960),
K Nakayasu, and
Y Watanabe, and
T Gotoh, and
T Kato, and
T Ishikawa, and
A Kanai
May 2009,
Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists),
K Nakayasu, and
Y Watanabe, and
T Gotoh, and
T Kato, and
T Ishikawa, and
A Kanai
January 2010,
Klinika oczna,
K Nakayasu, and
Y Watanabe, and
T Gotoh, and
T Kato, and
T Ishikawa, and
A Kanai
January 1997,
European journal of ophthalmology,
K Nakayasu, and
Y Watanabe, and
T Gotoh, and
T Kato, and
T Ishikawa, and
A Kanai
October 1988,
Ophthalmology,
K Nakayasu, and
Y Watanabe, and
T Gotoh, and
T Kato, and
T Ishikawa, and
A Kanai
January 2003,
Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde,
