BIOMAT Database Logo BIOMAT Database Logo

Immunohistochemical localization of somatostatin receptor sst2A in human rheumatoid synovium. 1999

A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Department of Immunology, Erasmus University, Rotterdam, The Netherlands. tenbokum@immu.fgg.eur.nl

OBJECTIVE To identify the somatostatin receptor-expressing cells in rheumatoid synovium using a recently developed antiserum directed against the somatostatin receptor subtype 2A (sst2A). METHODS We carried out immunohistochemical studies of synovial biopsies from 7 patients with rheumatoid arthritis (RA) and one non-RA patient, using a rabbit polyclonal antiserum directed against sst2A and monoclonal antibodies directed against phenotypic markers. RESULTS SSt2A was expressed by the endothelial cells of the synovial venules but also by a subset of synovial macrophages. CONCLUSIONS The identification of somatostatin receptors on macrophages, which are thought to be important effector cells in RA, may offer mechanistic insights into the potential therapeutic effect of somatostatin (analogs) in RA.

UI MeSH Term Description Entries
D007124 Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens. Antibody Enzyme Technique, Unlabeled,Enzyme Immunoassay,Enzyme-Labeled Antibody Technique,Immunoassay, Enzyme,Immunoperoxidase Techniques,Peroxidase-Antiperoxidase Complex Technique,Peroxidase-Labeled Antibody Technique,Antibody Enzyme Technic, Unlabeled,Enzyme-Labeled Antibody Technic,Immunoenzyme Technics,Immunoperoxidase Technics,Peroxidase-Antiperoxidase Complex Technic,Peroxidase-Labeled Antibody Technic,Antibody Technic, Enzyme-Labeled,Antibody Technic, Peroxidase-Labeled,Antibody Technics, Enzyme-Labeled,Antibody Technics, Peroxidase-Labeled,Antibody Technique, Enzyme-Labeled,Antibody Technique, Peroxidase-Labeled,Antibody Techniques, Enzyme-Labeled,Antibody Techniques, Peroxidase-Labeled,Enzyme Immunoassays,Enzyme Labeled Antibody Technic,Enzyme Labeled Antibody Technique,Enzyme-Labeled Antibody Technics,Enzyme-Labeled Antibody Techniques,Immunoassays, Enzyme,Immunoenzyme Technic,Immunoenzyme Technique,Immunoperoxidase Technic,Immunoperoxidase Technique,Peroxidase Antiperoxidase Complex Technic,Peroxidase Antiperoxidase Complex Technique,Peroxidase Labeled Antibody Technic,Peroxidase Labeled Antibody Technique,Peroxidase-Antiperoxidase Complex Technics,Peroxidase-Antiperoxidase Complex Techniques,Peroxidase-Labeled Antibody Technics,Peroxidase-Labeled Antibody Techniques,Technic, Enzyme-Labeled Antibody,Technic, Immunoenzyme,Technic, Immunoperoxidase,Technic, Peroxidase-Antiperoxidase Complex,Technic, Peroxidase-Labeled Antibody,Technics, Enzyme-Labeled Antibody,Technics, Immunoenzyme,Technics, Immunoperoxidase,Technics, Peroxidase-Antiperoxidase Complex,Technics, Peroxidase-Labeled Antibody,Technique, Enzyme-Labeled Antibody,Technique, Immunoenzyme,Technique, Immunoperoxidase,Technique, Peroxidase-Antiperoxidase Complex,Technique, Peroxidase-Labeled Antibody,Techniques, Enzyme-Labeled Antibody,Techniques, Immunoenzyme,Techniques, Immunoperoxidase,Techniques, Peroxidase-Antiperoxidase Complex,Techniques, Peroxidase-Labeled Antibody
D008833 Microcirculation The circulation of the BLOOD through the MICROVASCULAR NETWORK. Microvascular Blood Flow,Microvascular Circulation,Blood Flow, Microvascular,Circulation, Microvascular,Flow, Microvascular Blood,Microvascular Blood Flows,Microvascular Circulations
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D001172 Arthritis, Rheumatoid A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated. Rheumatoid Arthritis
D001345 Autoradiography The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed) Radioautography
D013583 Synovial Membrane The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID. Synovium,Membrana Synovialis Capsulae Articularis,Membrane, Synovial,Membranes, Synovial,Synovial Membranes

Related Publications

A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Immunohistochemical localization of somatostatin receptor sst2A in human pancreatic islets.
October 1998, The Journal of clinical endocrinology and metabolism,
A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Immunohistochemical localization of somatostatin receptors sst2A in human tumors.
July 1998, The American journal of pathology,
A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Immunohistochemical localization of somatostatin receptor SST2A in the rat pancreas.
June 1997, Endocrinology,
A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Immunohistochemical Localization of Somatostatin Receptor SST2A in the Rat Pancreas.
June 1997, Endocrinology,
A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Immunohistochemical localization of somatostatin receptor SST2A in the rat pancreas.
July 1997, Endocrinology,
A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Immunohistochemical detection of somatostatin receptor subtypes sst1 and sst2A in human somatostatin receptor positive tumors.
February 1999, The Journal of clinical endocrinology and metabolism,
A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Immunohistochemical localization of somatostatin receptor sst2A in human gut and lung tissue: possible implications for physiology and carcinogenesis.
April 2004, Annals of the New York Academy of Sciences,
A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Immunohistochemical determination of five somatostatin receptors in meningioma reveals frequent overexpression of somatostatin receptor subtype sst2A.
May 2000, Clinical cancer research : an official journal of the American Association for Cancer Research,
A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Immunocytochemical localization of somatostatin receptor sst2A in the rat spinal cord and dorsal root ganglia.
December 1998, The European journal of neuroscience,
A M ten Bokum, and M J Melief, and A Schonbrunn, and F van der Ham, and J Lindeman, and L J Hofland, and S W Lamberts, and P M van Hagen
Localization of the somatostatin receptor SST2A in rat brain using a specific anti-peptide antibody.
July 1996, The Journal of neuroscience : the official journal of the Society for Neuroscience,
Copied contents to your clipboard!