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The polyunsaturated fatty acids (PUFAs) of the omega 3 series are known to modulate adrenergic functions in ventricular myocytes. This study evaluated the influence of hypoxia duration and PUFA composition on the ability of cultured rat cardiomyocytes in producing alpha- and beta-adrenergic messengers (IPs and cAMP). After hypoxia (1.5, 2.5 or 3.5 h) followed by reoxygenation (1h). IP and cAMP production was induced by phenylephrine or isoproterenol stimulation, respectively. Hypoxia did not affect the basal level of messenger production in unstimulated cells, but decreased the cAMP production elicited by isoproterenol stimulation (up to 50%). The decrease in IP production after phenylephrine stimulation was observed only after long-term hypoxia duration close to irreversible cellular damages. The use of modified culture media supplemented with either arachidonic acid (AA) or docosahexaenoic acid (DHA) induced cardiomyocytes displaying either an arachidonic acid membrane profile (35% AA and 2% DHA in the phospholipids) or a docosahexaenoic acid membrane profile (15% AA and 20% DHA). These modifications did not alter the basal level of either messenger production in unstimulated cells nor the IP released after alpha-adrenergic stimulation. Conversely, the decrease in cAMP production was significantly more pronounced in docosahexaenoic acid-enriched cells than in arachidonic acid-enriched cells. This study suggests that hypoxia alters the beta-adrenergic messenger production, and that the alpha-system may balance the depression of the beta-system. The depression of the beta-adrenergic function induced by the incorporation of docosahexaenoic acid in membrane phospholipids may contribute to the beneficial effect of this fatty acid in the reperfused heart.
P Delerive, and
F Oudot, and
B Ponsard, and
S Talpin, and
J P Sergiel, and
C Cordelet, and
P Athias, and
A Grynberg
January 1995,
The American journal of physiology,
P Delerive, and
F Oudot, and
B Ponsard, and
S Talpin, and
J P Sergiel, and
C Cordelet, and
P Athias, and
A Grynberg
June 2005,
Nutrition, metabolism, and cardiovascular diseases : NMCD,
P Delerive, and
F Oudot, and
B Ponsard, and
S Talpin, and
J P Sergiel, and
C Cordelet, and
P Athias, and
A Grynberg
January 1998,
International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition,
P Delerive, and
F Oudot, and
B Ponsard, and
S Talpin, and
J P Sergiel, and
C Cordelet, and
P Athias, and
A Grynberg
February 1992,
The American journal of physiology,
P Delerive, and
F Oudot, and
B Ponsard, and
S Talpin, and
J P Sergiel, and
C Cordelet, and
P Athias, and
A Grynberg
November 1997,
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P Delerive, and
F Oudot, and
B Ponsard, and
S Talpin, and
J P Sergiel, and
C Cordelet, and
P Athias, and
A Grynberg
May 2005,
Journal of immunology (Baltimore, Md. : 1950),
P Delerive, and
F Oudot, and
B Ponsard, and
S Talpin, and
J P Sergiel, and
C Cordelet, and
P Athias, and
A Grynberg
January 1991,
Anticancer research,
P Delerive, and
F Oudot, and
B Ponsard, and
S Talpin, and
J P Sergiel, and
C Cordelet, and
P Athias, and
A Grynberg
March 2003,
Journal of agricultural and food chemistry,
P Delerive, and
F Oudot, and
B Ponsard, and
S Talpin, and
J P Sergiel, and
C Cordelet, and
P Athias, and
A Grynberg
July 2011,
Journal of neurochemistry,
P Delerive, and
F Oudot, and
B Ponsard, and
S Talpin, and
J P Sergiel, and
C Cordelet, and
P Athias, and
A Grynberg
July 1999,
Lipids,
