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Rat brain cytosol was applied to a heparin column and eluted with 0.9 M-NaCl. The total binding activity of [3H]inositol 1,4,5-trisphosphate to the eluate was increased about 6-fold compared with the original cytosol. When the eluate was mixed with a flow-through fraction from the heparin column, however, the activity returned to the original level, suggesting that the flow-through fraction contained an inhibitory factor(s) which prevented the binding. The factor(s) was purified by sequential column chromatography using gel permeation, a hydrophobic gel, and finally, a hydroxylapatite gel. Silver staining of sodium dedecyl sulfate gel electrophoresis of the sample thus purified showed a broad band located between the authentic molecular weight markers of 580 and 390 k. A carbohydrate staining method showed that the factor is a glycoprotein.
M Hirata, and
M Yoshida, and
T Kanematsu, and
H Takeuchi
February 1993,
Biochemistry,
M Hirata, and
M Yoshida, and
T Kanematsu, and
H Takeuchi
February 2005,
Proceedings of the National Academy of Sciences of the United States of America,
M Hirata, and
M Yoshida, and
T Kanematsu, and
H Takeuchi
November 1991,
The Journal of biological chemistry,
M Hirata, and
M Yoshida, and
T Kanematsu, and
H Takeuchi
August 1997,
The Biochemical journal,
M Hirata, and
M Yoshida, and
T Kanematsu, and
H Takeuchi
February 1999,
The Journal of biological chemistry,
M Hirata, and
M Yoshida, and
T Kanematsu, and
H Takeuchi
May 2002,
Proceedings of the National Academy of Sciences of the United States of America,
M Hirata, and
M Yoshida, and
T Kanematsu, and
H Takeuchi
March 1993,
Trends in pharmacological sciences,
M Hirata, and
M Yoshida, and
T Kanematsu, and
H Takeuchi
July 1992,
Neuroendocrinology,
M Hirata, and
M Yoshida, and
T Kanematsu, and
H Takeuchi
November 1997,
The Biochemical journal,
M Hirata, and
M Yoshida, and
T Kanematsu, and
H Takeuchi
July 1998,
Psychopharmacology,
