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By immunizing rabbits--tolerant against the bulk of normal human serum proteins--with highly purified non-suppressible insulin-like activity (NSILA-S), an antiserum was obtained which made possible the development of a double-antibody radioimmunoassay. Its sensitivity is about 30 pg NSILA-S per tube or 150 pg NSILA-S/ml. The specificity exceeds that of the bioassay used for comparison which is based in the stimulation by NSILA-S of 125IUDR incorporation into chicken fibroblasts in culture. The radioimmunoassay is sufficiently sensitive and specific to allow direct NSILA-S measurement in serum or plasma samples of humans and experimental animals. In human plasma samples NSILA-S levels, carrying between less than 0.15 and 25 ng/ml , were found to have an average of about 4 ng/ml. In rats higher levels were observed with a mean of 7.7 ng/ml in 4-week-old animals, increasing to about 60 ng/ml in 6-month-old rats. In fasting rats the NSILA-S plasma level is reduced. In acid-treated samples of plasma considerably higher NSILA-S amounts are found.
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June 1966,
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K Reber, and
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March 1979,
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K Reber, and
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March 1969,
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K Reber, and
R Liske
October 1984,
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K Reber, and
R Liske
November 1981,
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K Reber, and
R Liske
August 1967,
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K Reber, and
R Liske
March 1974,
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K Reber, and
R Liske
August 1977,
Acta endocrinologica,
K Reber, and
R Liske
August 1981,
Clinical endocrinology,
K Reber, and
R Liske
February 1964,
Schweizerische medizinische Wochenschrift,
