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A simple and sensitive fluorometric assay method for taurine (2-aminoethanesulfonic acid) has been developed. For the separation of taurine, high voltage paper electrophoresis subsequent to column chromatographic procedures was employed. Fluorescent product of taurine was yielded by spraying fluorescamine (4-phenylspiro [furan-2(3H), 1'-phthalan]-3, 3'-dione) and borate buffer on the paper, and the fluorescence was assayed spectro-fluorometrically after eluting with 50% ethanol. The linear relationship between the concentration of taurine and fluorescence developed was achieved over the concentration ranges of 0.5-10 nmoles, and the recoveries obtained were 90-100%. The specificity of this method for taurine was satisfactory and structural analogues involved in the metabolic pathway of taurine did not interfere with the assay. Examples for tissue levels of taurine in various organs of the rat as determined by this new method are also presented.
K Yoshikawa, and
K Kuriyama
August 1978,
Analytical biochemistry,
K Yoshikawa, and
K Kuriyama
January 1967,
Biochemical and biophysical research communications,
K Yoshikawa, and
K Kuriyama
May 1975,
Analytical biochemistry,
K Yoshikawa, and
K Kuriyama
February 1966,
Rinsho byori. The Japanese journal of clinical pathology,
K Yoshikawa, and
K Kuriyama
January 2020,
Methods in molecular biology (Clifton, N.J.),
K Yoshikawa, and
K Kuriyama
January 1972,
Laboratornoe delo,
K Yoshikawa, and
K Kuriyama
September 1969,
The Journal of laboratory and clinical medicine,
K Yoshikawa, and
K Kuriyama
August 1970,
Analytical biochemistry,
K Yoshikawa, and
K Kuriyama
January 1968,
Ukrains'kyi biokhimichnyi zhurnal,
K Yoshikawa, and
K Kuriyama
July 1978,
Analytical biochemistry,
