[Electron microscopic study of the structure of colonies of culture of Actinomyces parvullus producing actinomycin D]. 1976

P L Zaslavskaia, and V G Zhukov, and V D Kuznetsov, and E K Berezina

Ultrafine structure of the mycellium of the colonies of active and non-active variants of Actinomyces parvullus, producing actinomycin D was studied. Functional rebuilding of the cells of the substrate and aerial mycellium during the colony development in connection with synthesis and accumulation of the antibiotic by the culture was shown. The process of production and secretion of an amorphous material in the cells of the substrate mycellium of the active variant of Actinomyces parvullus was studied. It was supposed that the above process was associated with the antibiotic synthesis by the organism cells. The phenomenon of intrahyphal growth of the hyphae observed in both the active and inactive variants of the colonies was rather characteristic of the cells of the culture studied.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D003609 Dactinomycin A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015) Actinomycin,Actinomycin D,Meractinomycin,Cosmegen,Cosmegen Lyovac,Lyovac-Cosmegen,Lyovac Cosmegen,Lyovac, Cosmegen,LyovacCosmegen
D000190 Actinomyces A genus of gram-positive, rod-shaped bacteria whose organisms are nonmotile. Filaments that may be present in certain species are either straight or wavy and may have swollen or clubbed heads.
D046508 Culture Techniques Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types. Culture Technique,Technique, Culture,Techniques, Culture

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