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A simple method of isolation of highly purified prolactin from acetonated preparations of anterior hypophysial lobes is described. The new method permits to obtain higher (about 10-fold) yields of the hormone, as compared to those obtained using previously described methods. Prolactin was extracted by acid aqueous acetone and was subsequently purified of extract by fractionation with acetone and NaCl and by isoelectric precipitation. The final stage of the hormone purification involved gel-filtration through Sephadex G-200; prolactin yield was 400 microgram per 1 hypophysis. The lactogenic activity of the hormone is 14 MU/mg; the sequence of N-terminal amino acid residues of prolactin is as follows: NH2-Leu-Pro-Ile-x-Pro-Leu(?)-Gly-Ala-.
A A Bulatov, and
N A Demina, and
Iu A Pankov
December 1979,
Biochimica et biophysica acta,
A A Bulatov, and
N A Demina, and
Iu A Pankov
September 1971,
Biochemical and biophysical research communications,
A A Bulatov, and
N A Demina, and
Iu A Pankov
December 1985,
General and comparative endocrinology,
A A Bulatov, and
N A Demina, and
Iu A Pankov
May 1966,
The Journal of endocrinology,
A A Bulatov, and
N A Demina, and
Iu A Pankov
August 1978,
General and comparative endocrinology,
A A Bulatov, and
N A Demina, and
Iu A Pankov
October 1995,
Biokhimiia (Moscow, Russia),
A A Bulatov, and
N A Demina, and
Iu A Pankov
January 1979,
Biochemistry and experimental biology,
A A Bulatov, and
N A Demina, and
Iu A Pankov
December 1976,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
A A Bulatov, and
N A Demina, and
Iu A Pankov
January 1983,
Archives of biochemistry and biophysics,
A A Bulatov, and
N A Demina, and
Iu A Pankov
January 1977,
General and comparative endocrinology,
