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This study determines the effect of ischemia and reperfusion on energy-linked Ca2+ uptake by myocardial mitochondria. The left anterior descending coronary artery was occluded in 14 mature pigs for 2 hr. In seven animals the ligature was released and the ischemic zone reperfused for 2 additional hours. After sacrifice, mitochondrial function was measured in normal and reperfused or ischemic areas of the left ventricle, using a polarographic method. Mitochondria were prepared without EDTA by standard procedures and Ca2+ uptake measured by 45Ca2+ isotope tracer. Uptake of Ca2+ by mitochondria derived from ischemic myocardium is markedly impaired with or without phosphate. Reperfusion may accentuate this impairment. The presence of exogenous Ca2+ inhibits the ability of ischemic or reperfused mitochondria to phosphorylate ADP.
C F Peng, and
M L Murphy, and
J J Kane, and
K D Straub
January 1983,
Advances in myocardiology,
C F Peng, and
M L Murphy, and
J J Kane, and
K D Straub
August 1994,
Journal of molecular and cellular cardiology,
C F Peng, and
M L Murphy, and
J J Kane, and
K D Straub
January 1989,
Enzyme,
C F Peng, and
M L Murphy, and
J J Kane, and
K D Straub
June 1969,
Laboratory investigation; a journal of technical methods and pathology,
C F Peng, and
M L Murphy, and
J J Kane, and
K D Straub
June 1969,
Laboratory investigation; a journal of technical methods and pathology,
C F Peng, and
M L Murphy, and
J J Kane, and
K D Straub
January 1980,
Advances in myocardiology,
C F Peng, and
M L Murphy, and
J J Kane, and
K D Straub
January 1993,
Japanese circulation journal,
C F Peng, and
M L Murphy, and
J J Kane, and
K D Straub
October 1991,
Cardiovascular drugs and therapy,
C F Peng, and
M L Murphy, and
J J Kane, and
K D Straub
November 2001,
Perfusion,
C F Peng, and
M L Murphy, and
J J Kane, and
K D Straub
November 1996,
Circulation research,
