-transparent.png){kind=logo}
To study the nuclear organization and dynamics of nucleotide excision repair (NER), the endonuclease ERCC1/XPF (for excision repair cross complementation group 1/xeroderma pigmentosum group F) was tagged with green fluorescent protein and its mobility was monitored in living Chinese hamster ovary cells. In the absence of DNA damage, the complex moved freely through the nucleus, with a diffusion coefficient (15 +/- 5 square micrometers per second) consistent with its molecular size. Ultraviolet light-induced DNA damage caused a transient dose-dependent immobilization of ERCC1/XPF, likely due to engagement of the complex in a single repair event. After 4 minutes, the complex regained mobility. These results suggest (i) that NER operates by assembly of individual NER factors at sites of DNA damage rather than by preassembly of holocomplexes and (ii) that ERCC1/XPF participates in repair of DNA damage in a distributive fashion rather than by processive scanning of large genome segments.
A B Houtsmuller, and
S Rademakers, and
A L Nigg, and
D Hoogstraten, and
J H Hoeijmakers, and
W Vermeulen
August 2008,
Molecular and cellular biology,
A B Houtsmuller, and
S Rademakers, and
A L Nigg, and
D Hoogstraten, and
J H Hoeijmakers, and
W Vermeulen
July 2011,
DNA repair,
A B Houtsmuller, and
S Rademakers, and
A L Nigg, and
D Hoogstraten, and
J H Hoeijmakers, and
W Vermeulen
September 2009,
Cancer research,
A B Houtsmuller, and
S Rademakers, and
A L Nigg, and
D Hoogstraten, and
J H Hoeijmakers, and
W Vermeulen
April 2020,
PLoS genetics,
A B Houtsmuller, and
S Rademakers, and
A L Nigg, and
D Hoogstraten, and
J H Hoeijmakers, and
W Vermeulen
April 1998,
The Journal of biological chemistry,
A B Houtsmuller, and
S Rademakers, and
A L Nigg, and
D Hoogstraten, and
J H Hoeijmakers, and
W Vermeulen
September 2010,
Anticancer research,
A B Houtsmuller, and
S Rademakers, and
A L Nigg, and
D Hoogstraten, and
J H Hoeijmakers, and
W Vermeulen
September 2019,
Journal of medicinal chemistry,
A B Houtsmuller, and
S Rademakers, and
A L Nigg, and
D Hoogstraten, and
J H Hoeijmakers, and
W Vermeulen
July 2017,
The EMBO journal,
A B Houtsmuller, and
S Rademakers, and
A L Nigg, and
D Hoogstraten, and
J H Hoeijmakers, and
W Vermeulen
February 2015,
The EMBO journal,
A B Houtsmuller, and
S Rademakers, and
A L Nigg, and
D Hoogstraten, and
J H Hoeijmakers, and
W Vermeulen
May 2022,
Genes to cells : devoted to molecular & cellular mechanisms,
