Biomaterial Database

The substrate specificity of an aminopeptidase (neutral arylamidase) from pig brain. 1976

A Neidle, and A Lajtha

Neutral arylamidase of pig brain has been purified 235 fold. At this purification the major band on disc-gel electrophoresis is associated with the enzyme activity, although several minor components are also present. The arylamidase is -SH dependent and puromycin inhibited. It has activity toward lysyl and arginyl beta-naphthylamides as well as toward the beta-naphthylamides of a large variety of neutral amino acids. The enzyme is strongly substrate inhibited, making the relative order of reactivity to aminoacyl beta-naphthylamides dependent upon the substrate concentration chosen for comparison. It also shows activity toward certain di-, tri-, and oligopeptides. When more than one residue is cleaved, the release is sequential, starting from the amino terminus of the peptide. It appears therefore that pig brain neutral arylamidase is an aminooligopeptidase of broad specificity. We suggest that beta-naphthylamides are model substrates representing the N-terminal end of peptides with three or more residues. The properties of pig brain enzyme are similar in many respects to those previously isolated from rat and bovine brain and bovine pituitary.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D010455	Peptides	Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS.	Peptide,Polypeptide,Polypeptides
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D002384	Catalysis	The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.	Catalyses
D000626	Aminopeptidases	A subclass of EXOPEPTIDASES that act on the free N terminus end of a polypeptide liberating a single amino acid residue. EC 3.4.11.	Aminopeptidase
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D013552	Swine	Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).	Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog