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Effects of heparin on growth factor-induced mitogenic and proliferative responses of rat pulmonary artery smooth muscle cells. 1997

H L Wang, and S A Kilfeather, and G R Martin, and C P Page
Department of Pharmacology, China Medical University, Shenyang, China.

OBJECTIVE To investigate whether heparin inhibits growth factor-induced mitogenic and proliferative responses of the pulmonary arterial smooth muscle cells (PASMC). METHODS Rat PASMC were cultured in medium 199 containing 10% fetal bovine serum (FBS). Mitogenesis was monitored from [methyl-3H] thymidine ([methyl-3H] TdR) uptake and cell proliferation was monitored by cell counting. RESULTS FBS (1%), platelet-derived growth factor (PDGF, 50 micrograms.L-1), fibroblast growth factor (FGF, 50 micrograms.L-1) or interleukin 1 alpha (IL-1 alpha, 100 ng.L-1) alone induced both rat PASMC mitogenesis and proliferation. FBS (10%) and the combination of FBS (1%) with PDGF (50 micrograms.L-1), FGF (50 micrograms.L-1), or IL-1 alpha (100 ng.L-1) increased mitogenesis of rat PASMC. Heparin (100 mg.L-1) inhibited rat PASMC proliferation (28% +/- 6%) and thymidine incorporation (27% +/- 7%) induced by FBS (10%), and also significantly inhibited cell proliferation 25% +/- 6%, 27% +/- 7%, and 20% +/- 4%, and [methyl-3H] TdR incorporation 23% +/- 7%, 26% +/- 6%, 20% +/- 6% induced by FBS (1%) in combination with PDGF (50 micrograms.L-1), FGF (50 micrograms.L-1), or IL-1 alpha (100 ng.L-1) respectively. The PASMC viability was not affected by heparin. CONCLUSIONS Proliferation and mitogenesis of rat PASMC induced by PDGF, FGF, and IL-1 alpha was augmented by simultaneous exposure to FBS. Heparin produced an inhibition on the proliferation and mitogenesis of rat PASMC caused by these growth factors.

UI MeSH Term Description Entries
D007375 Interleukin-1 A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation. IL-1,Lymphocyte-Activating Factor,Epidermal Cell Derived Thymocyte-Activating Factor,Interleukin I,Macrophage Cell Factor,T Helper Factor,Epidermal Cell Derived Thymocyte Activating Factor,Interleukin 1,Lymphocyte Activating Factor
D009131 Muscle, Smooth, Vascular The nonstriated involuntary muscle tissue of blood vessels. Vascular Smooth Muscle,Muscle, Vascular Smooth,Muscles, Vascular Smooth,Smooth Muscle, Vascular,Smooth Muscles, Vascular,Vascular Smooth Muscles
D010982 Platelet-Derived Growth Factor Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. Platelet Derived Growth Factor,Factor, Platelet-Derived Growth,Growth Factor, Platelet-Derived
D011651 Pulmonary Artery The short wide vessel arising from the conus arteriosus of the right ventricle and conveying unaerated blood to the lungs. Arteries, Pulmonary,Artery, Pulmonary,Pulmonary Arteries
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D005346 Fibroblast Growth Factors A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family. DNA Synthesis Factor,Fibroblast Growth Factor,Fibroblast Growth Regulatory Factor,Growth Factor, Fibroblast,Growth Factors, Fibroblast
D006493 Heparin A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts. Heparinic Acid,alpha-Heparin,Heparin Sodium,Liquaemin,Sodium Heparin,Unfractionated Heparin,Heparin, Sodium,Heparin, Unfractionated,alpha Heparin
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats

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