High-pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling.
1999
K McDonald
Electron Microscope Lab, University of California, Berkeley, USA.
UI
MeSH Term
Description
Entries
D008854
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Electron Microscopy
D005615
Freezing
Liquids transforming into solids by the removal of heat.
Melting
D000818
Animals
Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.
Animal,Metazoa,Animalia
D000941
Antigens
Substances that are recognized by the immune system and induce an immune reaction.
Antigen
D014021
Tissue Preservation
The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).