-transparent.png){kind=logo}
It was recently found that the effect of an exercise-induced increase in muscle GLUT-4 on insulin-stimulated glucose transport is masked by a decreased responsiveness to insulin in glycogen-supercompensated muscle. We evaluated the role of hexosamines in this decrease in insulin responsiveness and found that UDP-N-acetyl hexosamine concentrations were not higher in glycogen-supercompensated muscles than in control muscles with a low glycogen content. We determined whether the smaller increase in glucose transport is due to translocation of fewer GLUT-4 to the cell surface with the 2-N-4-(1-azi-2,2,2-trifluroethyl)-benzoyl-1, 3-bis(D-mannose-4-yloxy)-2-propylamine (ATB-[2-3H]BMPA) photolabeling technique. The insulin-induced increase in GLUT-4 at the cell surface was no greater in glycogen-supercompensated exercised muscle than in muscles of sedentary controls and only 50% as great as in exercised muscles with a low glycogen content. We conclude that the decreased insulin responsiveness of glucose transport in glycogen-supercompensated muscle is not due to increased accumulation of hexosamine biosynthetic pathway end products and that the smaller increase in glucose transport is mediated by translocation of fewer GLUT-4 to the cell surface.
K Kawanaka, and
D H Han, and
L A Nolte, and
P A Hansen, and
A Nakatani, and
J O Holloszy
January 1994,
The American journal of physiology,
K Kawanaka, and
D H Han, and
L A Nolte, and
P A Hansen, and
A Nakatani, and
J O Holloszy
June 2000,
American journal of physiology. Endocrinology and metabolism,
K Kawanaka, and
D H Han, and
L A Nolte, and
P A Hansen, and
A Nakatani, and
J O Holloszy
January 1994,
Diabetologia,
K Kawanaka, and
D H Han, and
L A Nolte, and
P A Hansen, and
A Nakatani, and
J O Holloszy
July 1994,
The American journal of physiology,
K Kawanaka, and
D H Han, and
L A Nolte, and
P A Hansen, and
A Nakatani, and
J O Holloszy
February 1997,
The Histochemical journal,
K Kawanaka, and
D H Han, and
L A Nolte, and
P A Hansen, and
A Nakatani, and
J O Holloszy
December 1998,
Journal of applied physiology (Bethesda, Md. : 1985),
K Kawanaka, and
D H Han, and
L A Nolte, and
P A Hansen, and
A Nakatani, and
J O Holloszy
January 2003,
Methods in molecular medicine,
K Kawanaka, and
D H Han, and
L A Nolte, and
P A Hansen, and
A Nakatani, and
J O Holloszy
April 2003,
Journal of applied physiology (Bethesda, Md. : 1985),
K Kawanaka, and
D H Han, and
L A Nolte, and
P A Hansen, and
A Nakatani, and
J O Holloszy
October 1999,
The American journal of physiology,
K Kawanaka, and
D H Han, and
L A Nolte, and
P A Hansen, and
A Nakatani, and
J O Holloszy
February 2015,
American journal of physiology. Endocrinology and metabolism,
