BIOMAT Database Logo BIOMAT Database Logo

Brainstem projections to the ventromedial medulla in cat: retrograde transport horseradish peroxidase and immunohistochemical studies. 1999

Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
Department of Psychiatry, School of Medicine, University of California, Los Angeles, USA.

Stimulation of the nucleus magnocellularis (NMC) of the medulla produces changes in locomotion, muscle tone, heart rate, and blood pressure. Glutamatergic input has been found to modulate muscle tone, whereas cholinergic input has been found to mediate cardiovascular changes produced by stimulation of the NMC. The current study was designed to identify the brainstem afferents to NMC by using retrograde transport of wheat germ agglutinin and horseradish peroxidase (WGA-HRP) combined with glutamate and choline acetyltransferase (ChAT) immunohistochemical and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemical techniques. Fifty nanoliters of 2.5% WGA-HRP were microinjected into the NMC in the cat. A heavy density of WGA-HRP-labeled neurons was found in the ipsilateral mesencephalic reticular formation (MRF), periaqueductal gray, Kolliker-Fuse nucleus, and pontis centralis caudalis (PoC), in the contralateral pontis centralis oralis (PoO), and bilaterally in the nucleus paragigantocellularis lateralis. A moderate density of retrogradely labeled neurons was found in the ipsilateral side of the nuclei parvocellularis, retrorubral (RRN), PoO, and vestibular complex, in the contralateral PoC and nucleus gigantocellularis, and bilaterally in the inferior vestibular nucleus. Retrograde HRP/glutamate-positive cells could be found throughout the brainstem, with a high percentage in RRN, PoO, PoC, and MRF. Double-labeled WGA-HRP/ChAT neurons were found in the pedunculopontine nucleus. Double-labeled WGA-HRP/NADPH-d-positive neurons could be seen in many nuclei of the brainstem, although the number of labeled neurons was small. The dense glutamatergic projections to the NMC support the hypothesis that rostral brainstem glutamatergic mechanisms regulate muscle activity and locomotor coordination via the NMC, whereas the pontine cholinergic projections to the NMC participate in cardiovascular regulation.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008526 Medulla Oblongata The lower portion of the BRAIN STEM. It is inferior to the PONS and anterior to the CEREBELLUM. Medulla oblongata serves as a relay station between the brain and the spinal cord, and contains centers for regulating respiratory, vasomotor, cardiac, and reflex activities. Accessory Cuneate Nucleus,Ambiguous Nucleus,Arcuate Nucleus of the Medulla,Arcuate Nucleus-1,External Cuneate Nucleus,Lateral Cuneate Nucleus,Nucleus Ambiguus,Ambiguus, Nucleus,Arcuate Nucleus 1,Arcuate Nucleus-1s,Cuneate Nucleus, Accessory,Cuneate Nucleus, External,Cuneate Nucleus, Lateral,Medulla Oblongatas,Nucleus, Accessory Cuneate,Nucleus, Ambiguous,Nucleus, External Cuneate,Nucleus, Lateral Cuneate
D009252 NADPH Dehydrogenase A flavoprotein that reversibly oxidizes NADPH to NADP and a reduced acceptor. EC 1.6.99.1. NADP Dehydrogenase,NADP Diaphorase,NADPH Diaphorase,Old Yellow Enzyme,TPN Diaphorase,Dehydrogenase, NADP,Dehydrogenase, NADPH,Diaphorase, NADP,Diaphorase, NADPH,Diaphorase, TPN,Enzyme, Old Yellow
D001931 Brain Mapping Imaging techniques used to colocalize sites of brain functions or physiological activity with brain structures. Brain Electrical Activity Mapping,Functional Cerebral Localization,Topographic Brain Mapping,Brain Mapping, Topographic,Functional Cerebral Localizations,Mapping, Brain,Mapping, Topographic Brain
D002415 Cats The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801) Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D002795 Choline O-Acetyltransferase An enzyme that catalyzes the formation of acetylcholine from acetyl-CoA and choline. EC 2.3.1.6. Choline Acetylase,Choline Acetyltransferase,Acetylase, Choline,Acetyltransferase, Choline,Choline O Acetyltransferase,O-Acetyltransferase, Choline
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001692 Biological Transport The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments. Transport, Biological,Biologic Transport,Transport, Biologic
D018998 Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate The lectin wheatgerm agglutinin conjugated to the enzyme HORSERADISH PEROXIDASE. It is widely used for tracing neural pathways. WGA-HRP,Wheat Germ Agglutinin Horseradish Peroxidase Conjugate

Related Publications

Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
The vestibulothalamic projections in the cat studied by retrograde axonal transport of horseradish peroxidase.
January 1980, Experimental brain research,
Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
Projections to the ventral surface of the cat brainstem demonstrated by horseradish peroxidase.
May 1979, Neuroscience letters,
Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
Pallido-cortical projections in the cat studied by means of the horseradish peroxidase retrograde transport technique.
April 1982, Neuroscience letters,
Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
Thalamic projections to the cortical gustatory area in the cat studied by retrograde axonal transport of horseradish peroxidase.
January 1989, Journal fur Hirnforschung,
Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
Cortical projections to the periaqueductal grey in the cat: a retrograde horseradish peroxidase study.
October 1984, Neuroscience letters,
Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
The afferent projections to the centrum medianum of the cat as demonstrated by retrograde transport of horseradish peroxidase.
February 1980, Brain research,
Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
[Cerebellar corticovestibular and vestibulocerebellar projections studied by the retrograde and anterograde transport of horseradish peroxidase in the cat].
June 1987, Kaibogaku zasshi. Journal of anatomy,
Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
Laminar origins of spinothalamic projections in the cat as determined by the retrograde transport of horseradish peroxidase.
November 1978, The Journal of comparative neurology,
Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
Thalamic projections of the vestibular nuclei in the cat as revealed by retrograde transport of horseradish peroxidase.
September 1978, Neuroscience letters,
Y Y Lai, and J R Clements, and X Y Wu, and T Shalita, and J P Wu, and J S Kuo, and J M Siegel
Projections from the inferior olive to the cerebellar nuclei in the cat demonstrated by retrograde transport of horseradish peroxidase.
July 1977, Brain research,
Copied contents to your clipboard!