Expression of serotonin receptor 2c in rat type II pneumocytes. 1999

D Wang, and M Post, and E Cutz
Division of Pathology, Department of Pediatric Laboratory Medicine, The Research Institute, MRC Group on Lung Development, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.

Serotonin (5-hydroxytryptamine [5-HT]) is a multifunctional amine with wide occurrence in both neural and non-neural tissues, including the lung. The diverse responses to 5-HT are elicited through activation of different 5-HT receptor subtypes. We report the expression and localization of 5-HT receptor 2c subtype (5-HT2c-R) in rat lungs using reverse transcriptase-polymerase chain reaction, nonisotopic in situ hybridization (NISH), and immunohistochemistry. At the messenger RNA (mRNA) level, signal corresponding to approximately 430 base pairs was detected in whole-lung tissue extracts as well as in cultures of isolated alveolar type II cells from fetal and adult rat lung. Using antisense RNA probe for 5-HT2c-R, NISH showed strong positive signal in type II cells. The expression of mRNA signal differed between fetal and adult rat type II cells, with weak, predominantly perinuclear localization in the former and strong cytoplasmic localization in the latter. Immunohistochemistry, using specific monoclonal antibody against 5-HT2c-R, showed perinuclear localization in fetal type II cells; whereas in adult type II cells 5-HT2c-R immunoreactivity was confined mostly to the plasma membrane, as demonstrated by laser confocal microscopy. Identification of 5-HT2c-R expression in alveolar type II cells suggests an important role for this amine in modulating the function of these cells. The differences in cell domain localization between fetal and adult type II cells could indicate developmental regulation of 5-HT2c-R expression in the lung.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D011650 Pulmonary Alveoli Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place. Alveoli, Pulmonary,Alveolus, Pulmonary,Pulmonary Alveolus
D011985 Receptors, Serotonin Cell-surface proteins that bind SEROTONIN and trigger intracellular changes which influence the behavior of cells. Several types of serotonin receptors have been recognized which differ in their pharmacology, molecular biology, and mode of action. 5-HT Receptor,5-HT Receptors,5-Hydroxytryptamine Receptor,5-Hydroxytryptamine Receptors,Receptors, Tryptamine,Serotonin Receptor,Serotonin Receptors,Tryptamine Receptor,Tryptamine Receptors,Receptors, 5-HT,Receptors, 5-Hydroxytryptamine,5 HT Receptor,5 HT Receptors,5 Hydroxytryptamine Receptor,5 Hydroxytryptamine Receptors,Receptor, 5-HT,Receptor, 5-Hydroxytryptamine,Receptor, Serotonin,Receptor, Tryptamine,Receptors, 5 HT,Receptors, 5 Hydroxytryptamine
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012701 Serotonin A biochemical messenger and regulator, synthesized from the essential amino acid L-TRYPTOPHAN. In humans it is found primarily in the central nervous system, gastrointestinal tract, and blood platelets. Serotonin mediates several important physiological functions including neurotransmission, gastrointestinal motility, hemostasis, and cardiovascular integrity. Multiple receptor families (RECEPTORS, SEROTONIN) explain the broad physiological actions and distribution of this biochemical mediator. 5-HT,5-Hydroxytryptamine,3-(2-Aminoethyl)-1H-indol-5-ol,Enteramine,Hippophaine,Hydroxytryptamine,5 Hydroxytryptamine
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats
D017403 In Situ Hybridization A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes. Hybridization in Situ,Hybridization, In Situ,Hybridizations, In Situ,In Situ Hybridizations
D044405 Receptor, Serotonin, 5-HT2C A serotonin receptor subtype found primarily in the CENTRAL NERVOUS SYSTEM and the CHOROID PLEXUS. This receptor subtype is believed to mediate the anorectic action of SEROTONIN, while selective antagonists of the 5-HT2C receptor appear to induce ANXIETY. Several isoforms of this receptor subtype exist, due to adenine deaminase editing of the receptor mRNA. Serotonin 2C Receptor,5-HT(2C) Receptor,5-HT2C Receptor,5-Hydroxytryptamine Type 2C Receptor,5-Hydroxytryptamine Type 2C Receptors,Receptor, 5-Hydroxytryptamine Type 2C,5 HT2C Receptor,5 Hydroxytryptamine Type 2C Receptor,5 Hydroxytryptamine Type 2C Receptors,Receptor, 5 Hydroxytryptamine Type 2C,Receptor, 5-HT2C,Receptor, Serotonin 2C

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