-transparent.png){kind=logo}
Phospholipase C (PLC) is the key enzyme in the phototransduction cascade of invertebrate rhabdomeric photoreceptors. In addition to 130 kDa PLC, a 95 kDa protein recognized by antibody against the catalytic site of PLC was found in the squid retina. The PLC-like 95 kDa protein (95 kDa PLC) was produced from 130 kDa PLC by an intrinsic protease in the presence of calcium. The 130 kDa PLC was stimulated by the active form of Gq-class G-protein alpha (Gq alpha), but the 95 kDa PLC was not, although their PLC activity was similar. A 35 kDa fragment, the counterpart of 95 kDa PLC, was not recognized by antibodies against catalytic site or N-terminal site of the 130 kDa PLC, indicating that the cleavage site is on the C-terminal side beyond the catalytic site. In the presence of a large excess of the 35 kDa fragment, 95 kDa PLC was stimulated by Gq alpha to a similar extent as intact 130 kDa PLC. These results indicate that the C-terminal polypeptide of PLC is necessary for regulation of its enzyme activity by Gq alpha. The uncoupling of PLC from Gq alpha, caused by limited proteolysis, is therefore a candidate regulatory mechanism of the phototransduction cascade in rhabdomeric photoreceptors.
T Suzuki, and
K Narita, and
A Terakita, and
E Takai, and
K Nagai, and
Y Kito, and
Y Tsukahara
November 1992,
Biochemical and biophysical research communications,
T Suzuki, and
K Narita, and
A Terakita, and
E Takai, and
K Nagai, and
Y Kito, and
Y Tsukahara
May 1995,
Biochemical Society transactions,
T Suzuki, and
K Narita, and
A Terakita, and
E Takai, and
K Nagai, and
Y Kito, and
Y Tsukahara
June 2001,
The Journal of biological chemistry,
T Suzuki, and
K Narita, and
A Terakita, and
E Takai, and
K Nagai, and
Y Kito, and
Y Tsukahara
December 1989,
Trends in pharmacological sciences,
T Suzuki, and
K Narita, and
A Terakita, and
E Takai, and
K Nagai, and
Y Kito, and
Y Tsukahara
January 1995,
The Journal of biological chemistry,
T Suzuki, and
K Narita, and
A Terakita, and
E Takai, and
K Nagai, and
Y Kito, and
Y Tsukahara
April 1991,
Biochemical Society transactions,
T Suzuki, and
K Narita, and
A Terakita, and
E Takai, and
K Nagai, and
Y Kito, and
Y Tsukahara
December 1992,
Trends in biochemical sciences,
T Suzuki, and
K Narita, and
A Terakita, and
E Takai, and
K Nagai, and
Y Kito, and
Y Tsukahara
May 2004,
The Journal of biological chemistry,
T Suzuki, and
K Narita, and
A Terakita, and
E Takai, and
K Nagai, and
Y Kito, and
Y Tsukahara
March 1990,
The American review of respiratory disease,
T Suzuki, and
K Narita, and
A Terakita, and
E Takai, and
K Nagai, and
Y Kito, and
Y Tsukahara
July 1996,
Nature,
