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Whole-cell patch clamp recordings in combination with direct control and measurements of O2 tension (pO2) in bath solution were used to determine the sensitivity of Ca2+ channels of cultured hippocampal neurones to hypoxia in glucose free solution. In all tested neurones, a lowering of pO2 to 4/50 mmHg did not induce changes either in magnitude, kinetics or voltage-current relations of total Ca2+ currents, which composed mainly from two types, L-type (64%) and N-type (31%) components. Hypoxia only induced a delay of Ca2+ current run-down about 27.5% and 39% at 50 and 4 mmHg pO2 respectively that presumably depended on changes in cytoplasmic channel-modulatory metabolites. The obtained results demonstrate that Ca2+ channel molecules in cultured hippocampal neurones are themselves insensitive to short-lasting (10-20 min) oxygen and glucose deprivation, and that they are not a principal target for hypoxic influences on hippocampal function.
V M Shkryl, and
L M Nikolaenko, and
P G Kostyuk, and
E A Lukyanetz
December 2001,
Neuroreport,
V M Shkryl, and
L M Nikolaenko, and
P G Kostyuk, and
E A Lukyanetz
January 2002,
British journal of pharmacology,
V M Shkryl, and
L M Nikolaenko, and
P G Kostyuk, and
E A Lukyanetz
November 1994,
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V M Shkryl, and
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E A Lukyanetz
June 1991,
Brain research,
V M Shkryl, and
L M Nikolaenko, and
P G Kostyuk, and
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November 1995,
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V M Shkryl, and
L M Nikolaenko, and
P G Kostyuk, and
E A Lukyanetz
September 1995,
Endocrinology,
V M Shkryl, and
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P G Kostyuk, and
E A Lukyanetz
March 1987,
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V M Shkryl, and
L M Nikolaenko, and
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E A Lukyanetz
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V M Shkryl, and
L M Nikolaenko, and
P G Kostyuk, and
E A Lukyanetz
May 1991,
Brain research,
V M Shkryl, and
L M Nikolaenko, and
P G Kostyuk, and
E A Lukyanetz
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Free radical biology & medicine,
