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OBJECTIVE To study the effect of mitoxantrone (Mit) on DNA polymerases of tumor cells. METHODS DNA polymerases of Ehrlich ascites carcinoma cells were isolated by phosphocellulose column chromatography. The effects of Mit on DNA polymerase alpha, beta, and gamma were detected by method of K Ono. RESULTS Mit inhibited DNA polymerase alpha, beta, and gamma, IC50 values were 11.9, 6.5, and 11.9 mumol.L-1, and Ki 1.86, 2.22, and 2.05 mumol.L-1, respectively. The inhibitory mode of Mit on DNA polymerase alpha, beta, and gamma was competitive. CONCLUSIONS Mit is a strong inhibitor on DNA polymerase alpha, beta, and gamma. The inhibitory mode was competition with respect to template DNA.
L J Xian, and
H X Li, and
Z C Liu, and
Q C Pan
July 1965,
Canadian journal of biochemistry,
L J Xian, and
H X Li, and
Z C Liu, and
Q C Pan
July 1984,
Biokhimiia (Moscow, Russia),
L J Xian, and
H X Li, and
Z C Liu, and
Q C Pan
March 2013,
Bulletin of experimental biology and medicine,
L J Xian, and
H X Li, and
Z C Liu, and
Q C Pan
January 1965,
Voprosy meditsinskoi khimii,
L J Xian, and
H X Li, and
Z C Liu, and
Q C Pan
November 1975,
Biochemical and biophysical research communications,
L J Xian, and
H X Li, and
Z C Liu, and
Q C Pan
March 1971,
Biochemical and biophysical research communications,
L J Xian, and
H X Li, and
Z C Liu, and
Q C Pan
February 1968,
Gan,
L J Xian, and
H X Li, and
Z C Liu, and
Q C Pan
August 1984,
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,
L J Xian, and
H X Li, and
Z C Liu, and
Q C Pan
January 1972,
Oncology,
L J Xian, and
H X Li, and
Z C Liu, and
Q C Pan
September 1969,
British journal of cancer,
