Tribenoside in a dose of 100 mg/kg i.p. significantly accelerates the regeneration of puncture wounds in the caudal membrane of Amblystoma mexicanum. A dose of 200 mg/kg exerts a less-pronounced vulnerary effect and is occasionally toxic. Tribenoside also stimulates regeneration in this species when added to bath fluid in a concentration of 1:400,000 (exposure for 6 h daily). A concentration of 1:200,000 is less effective and has a slightly irritant effect on the tissue. 14 days after operation, the rate of mitosis in the regenerative blastema is distinctly greater than that demonstrable in the normal integument of the axolotl. The number of cell divisions in the regenerating tissue is not significantly altered by tribenoside in a concentration of 1:400,000. The possible involvement of other mechanisms in the stimulated regeneration observed after treatment with tribenoside is discussed.