BIOMAT Database Logo BIOMAT Database Logo

Human granulosa cells express integrin alpha2 and collagen type IV: possible involvement of collagen type IV in granulosa cell luteinization. 1999

S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.

Previously, it has been shown that integrin alpha6beta1 expressed on human granulosa cells regulates luteinization in co-operation with its ligand, laminin. In this study, integrin alpha2 was immunohistochemically demonstrated to be expressed on granulosa and large luteal cells. It was also detected on luteinizing theca interna cells after ovulation. Immunoreactive collagen type IV, which is one of the ligands for integrin alpha2beta1, was detected around granulosa cells in the pre-ovulatory follicles and its expression was rapidly increased during ovulation. By flow cytometry, collagen type IV was detected on the cell surface of luteinizing granulosa cells isolated from pre-ovulatory follicles, confirming the physiological interaction between granulosa cells and collagen type IV. Collagen type IV in follicular fluid was positively related with progesterone concentration. In 4-day cultures of granulosa cells, collagen type IV in the media was significantly increased by human chorionic gonadotrophin (HCG). The progesterone production was significantly attenuated when granulosa cells were cultured on collagen type IV-coated dishes, suggesting that collagen type IV suppresses granulosa cell luteinization. These findings show that collagen type IV, a ligand for integrin alpha2beta1, is rapidly produced around luteinizing granulosa cells during ovulation, probably under the control of luteinizing hormone (LH) and suggest that collagen type IV is a new parameter and/or regulator of granulosa cell luteinization in the periovulatory phases.

UI MeSH Term Description Entries
D008184 Luteal Cells PROGESTERONE-producing cells in the CORPUS LUTEUM. The large luteal cells derive from the GRANULOSA CELLS. The small luteal cells derive from the THECA CELLS. Lutein Cells,Granulosa-Luteal Cells,Granulosa-Lutein Cells,Large Luteal Cells,Small Luteal Cells,Theca-Luteal cells,Theca-Lutein Cells,Cell, Granulosa-Luteal,Cell, Granulosa-Lutein,Cell, Large Luteal,Cell, Luteal,Cell, Lutein,Cell, Small Luteal,Cell, Theca-Lutein,Cells, Granulosa-Luteal,Cells, Granulosa-Lutein,Cells, Large Luteal,Cells, Luteal,Cells, Lutein,Cells, Small Luteal,Cells, Theca-Lutein,Granulosa Luteal Cells,Granulosa Lutein Cells,Granulosa-Luteal Cell,Granulosa-Lutein Cell,Large Luteal Cell,Luteal Cell,Luteal Cell, Large,Luteal Cell, Small,Luteal Cells, Large,Luteal Cells, Small,Lutein Cell,Small Luteal Cell,Theca Luteal cells,Theca Lutein Cells,Theca-Luteal cell,Theca-Lutein Cell,cell, Theca-Luteal,cells, Theca-Luteal
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006107 Granulosa Cells Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH). Cell, Granulosa,Cells, Granulosa,Granulosa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015703 Antigens, CD Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation. CD Antigen,Cluster of Differentiation Antigen,Cluster of Differentiation Marker,Differentiation Antigens, Leukocyte, Human,Leukocyte Differentiation Antigens, Human,Cluster of Differentiation Antigens,Cluster of Differentiation Markers,Antigen Cluster, Differentiation,Antigen, CD,CD Antigens,Differentiation Antigen Cluster,Differentiation Marker Cluster,Marker Cluster, Differentiation

Related Publications

S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
Bovine granulosa cells express extracellular matrix proteins and their regulators during luteinization in culture.
January 1996, Reproduction, fertility, and development,
S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
Type IV collagen-binding proteins of neutrophils: possible involvement of L-selectin in the neutrophil binding to type IV collagen.
January 1996, Blood,
S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
Intraovarian control of granulosa cell luteinization.
November 1982, Endocrinologia experimentalis,
S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
Integrin-mediated interactions between primary/T-sv40 immortalized human glomerular epithelial cells and type IV collagen.
March 1996, Laboratory investigation; a journal of technical methods and pathology,
S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
[Integrin beta1 mediates hepatocellular carcinoma cells adhesion & chemotaxis to type IV collagen].
October 2004, Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi,
S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
[Integrin alpha3beta1 mediates hepatocellular carcinoma cell adhesion and chemotaxis to type IV collagen].
June 2003, Zhonghua yi xue za zhi,
S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
Glycosylation modulates melanoma cell α2β1 and α3β1 integrin interactions with type IV collagen.
August 2014, The Journal of biological chemistry,
S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
Glomerular expression of alpha2(IV) and alpha5(IV) chains of type IV collagen in patients with IgA nephropathy.
May 2002, Nephron,
S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
Involvement of the cytoskeleton components in the secretion of luteinization stimulator by porcine granulosa cells.
December 1991, Endocrine regulations,
S Yamada, and H Fujiwara, and T Honda, and T Higuchi, and T Nakayama, and T Inoue, and M Maeda, and S Fujii
Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization.
June 1994, FEBS letters,
Copied contents to your clipboard!