BIOMAT Database Logo BIOMAT Database Logo

Stress tolerance in doughs of Saccharomyces cerevisiae trehalase mutants derived from commercial Baker's yeast. 1999

J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
National Food Research Institute, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. shimaj@nfri.affrc.go.jp

Accumulation of trehalose is widely believed to be a critical determinant in improving the stress tolerance of the yeast Saccharomyces cerevisiae, which is commonly used in commercial bread dough. To retain the accumulation of trehalose in yeast cells, we constructed, for the first time, diploid homozygous neutral trehalase mutants (Deltanth1), acid trehalase mutants (Deltaath1), and double mutants (Deltanth1 ath1) by using commercial baker's yeast strains as the parent strains and the gene disruption method. During fermentation in a liquid fermentation medium, degradation of intracellular trehalose was inhibited with all of the trehalase mutants. The gassing power of frozen doughs made with these mutants was greater than the gassing power of doughs made with the parent strains. The Deltanth1 and Deltaath1 strains also exhibited higher levels of tolerance of dry conditions than the parent strains exhibited; however, the Deltanth1 ath1 strain exhibited lower tolerance of dry conditions than the parent strain exhibited. The improved freeze tolerance exhibited by all of the trehalase mutants may make these strains useful in frozen dough.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D002245 Carbon Dioxide A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals. Carbonic Anhydride,Anhydride, Carbonic,Dioxide, Carbon
D005285 Fermentation Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID. Fermentations
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D014198 Trehalase An enzyme that catalyzes the conversion of alpha,alpha-trehalose and water to D-glucose. EC 3.2.1.28.
D014199 Trehalose
D017353 Gene Deletion A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus. Deletion, Gene,Deletions, Gene,Gene Deletions
D018869 Heat-Shock Response A sequence of responses that occur when an organism is exposed to excessive heat. In humans, an increase in skin temperature triggers muscle relaxation, sweating, and vasodilation. Heat-Shock Reaction,Heat Shock,Heat Shock Stress,Heat Stress,Heat-Stress Reaction,Heat-Stress Response,Heat Shock Reaction,Heat Shock Response,Heat Shock Stresses,Heat Shocks,Heat Stress Reaction,Heat Stress Response,Heat Stresses,Heat-Shock Reactions,Heat-Shock Responses,Heat-Stress Reactions,Heat-Stress Responses,Shock, Heat,Stress, Heat,Stress, Heat Shock
D019649 Food Industry The industry concerned with processing, preparing, preserving, distributing, and serving of foods and beverages. Food Industries,Industries, Food,Industry, Food

Related Publications

J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
Superoxide dismutase null mutants of baker's yeast, Saccharomyces cerevisiae.
January 2002, Methods in enzymology,
J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
Stress-tolerance of baker's-yeast (Saccharomyces cerevisiae) cells: stress-protective molecules and genes involved in stress tolerance.
May 2009, Biotechnology and applied biochemistry,
J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
Transcriptomic analysis of the heat stress response for a commercial baker's yeast Saccharomyces cerevisiae.
February 2018, Genes & genomics,
J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
[Construction and stress tolerance of trehalase mutant in Saccharomyces cerevisiae].
October 2008, Wei sheng wu xue bao = Acta microbiologica Sinica,
J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
Lysine-overproducing mutants of Saccharomyces cerevisiae baker's yeast isolated in continuous culture.
December 1997, Applied and environmental microbiology,
J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
Phenotypic features of trehalase mutants in Saccharomyces cerevisiae.
March 1995, FEBS letters,
J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
Disruption of the CAR1 gene encoding arginase enhances freeze tolerance of the commercial baker's yeast Saccharomyces cerevisiae.
January 2003, Applied and environmental microbiology,
J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
Triacylglycerol synthesis in lipid particles from baker's yeast (Saccharomyces cerevisiae).
July 1978, Biochimica et biophysica acta,
J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
Triacylglycerol lipase activity in baker's yeast (Saccharomyces cerevisiae).
March 1976, Biochimica et biophysica acta,
J Shima, and A Hino, and C Yamada-Iyo, and Y Suzuki, and R Nakajima, and H Watanabe, and K Mori, and H Takano
Synthesis of trehalose by baker's yeast (Saccharomyces cerevisiae).
September 1962, Archives of biochemistry and biophysics,
Copied contents to your clipboard!