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The taxonomic position of the nitrogen-fixing rice isolate A15, previously classified as Alcaligenes faecalis, was reinvestigated. On the basis of its small subunit ribosomal RNA (16S rRNA) sequence this strain identifies as Pseudomonas stutzeri. Phenotyping and fatty acid profiling confirm this result. DNA:DNA hybridisations, using the optical renaturation rate method, between strain A15 and Pseudomonas stutzeri LMG 11199T revealed a mean DNA-binding of 77%. The identification was further corroborated by comparative sequence analysis of the oprF gene, which encodes the major outer membrane protein of rRNA homology group I pseudomonads. Furthermore we determined the nifH sequence of this strain and of two putative diazotrophic Pseudomonas spp. and made a comparative analysis with sequences of other diazotrophs. These Pseudomonas NifH sequences cluster with NifH sequences isolated from the rice rhizosphere by PCR and of proteobacteria from the beta and gamma subclasses.
H Vermeiren, and
A Willems, and
G Schoofs, and
R de Mot, and
V Keijers, and
W Hai, and
J Vanderleyden
April 2017,
Archives of microbiology,
H Vermeiren, and
A Willems, and
G Schoofs, and
R de Mot, and
V Keijers, and
W Hai, and
J Vanderleyden
November 2003,
Applied and environmental microbiology,
H Vermeiren, and
A Willems, and
G Schoofs, and
R de Mot, and
V Keijers, and
W Hai, and
J Vanderleyden
August 2003,
Microbiology (Reading, England),
H Vermeiren, and
A Willems, and
G Schoofs, and
R de Mot, and
V Keijers, and
W Hai, and
J Vanderleyden
July 2011,
Journal of bacteriology,
H Vermeiren, and
A Willems, and
G Schoofs, and
R de Mot, and
V Keijers, and
W Hai, and
J Vanderleyden
August 2013,
Genome announcements,
H Vermeiren, and
A Willems, and
G Schoofs, and
R de Mot, and
V Keijers, and
W Hai, and
J Vanderleyden
April 2012,
Applied and environmental microbiology,
H Vermeiren, and
A Willems, and
G Schoofs, and
R de Mot, and
V Keijers, and
W Hai, and
J Vanderleyden
November 1983,
Plasmid,
H Vermeiren, and
A Willems, and
G Schoofs, and
R de Mot, and
V Keijers, and
W Hai, and
J Vanderleyden
January 2013,
Genome announcements,
H Vermeiren, and
A Willems, and
G Schoofs, and
R de Mot, and
V Keijers, and
W Hai, and
J Vanderleyden
December 2006,
Microbiology (Reading, England),
H Vermeiren, and
A Willems, and
G Schoofs, and
R de Mot, and
V Keijers, and
W Hai, and
J Vanderleyden
January 2009,
Microbiological research,
