-transparent.png){kind=logo}
Mammalian DNA polymerases alpha and beta lack 3' exonuclease activity and are unable to edit errors after DNA synthesis. However, editing exonucleases can be functions of separate polypeptides. We isolated a widely distributed DNA-specific 3' exonuclease from rabbit liver nuclei, sequenced tryptic peptides by mass spectrometry, and identified the corresponding human open reading frame. The protein expressed from the cloned human sequence exhibits 3' exonuclease activity. The human clone shares sequence homology with the editing function of the Escherichia coli DNA polymerase III holoenzyme, i.e., the DnaQ/MutD protein, and weakly with the editing 3' exonuclease domain of eukaryotic DNA polymerase epsilon. The gene maps to human chromosome 3p21.2-21.3. In a reconstituted human DNA repair system containing DNA polymerase beta and DNA ligase III-XRCC1, accurate rejoining of a 3' mismatched base residue at a single-strand break is dependent on addition of the exonuclease.
M Höss, and
P Robins, and
T J Naven, and
D J Pappin, and
J Sgouros, and
T Lindahl
April 1983,
Proceedings of the National Academy of Sciences of the United States of America,
M Höss, and
P Robins, and
T J Naven, and
D J Pappin, and
J Sgouros, and
T Lindahl
January 1988,
Molecular & general genetics : MGG,
M Höss, and
P Robins, and
T J Naven, and
D J Pappin, and
J Sgouros, and
T Lindahl
July 1986,
Journal of molecular biology,
M Höss, and
P Robins, and
T J Naven, and
D J Pappin, and
J Sgouros, and
T Lindahl
October 1986,
Molecular & general genetics : MGG,
M Höss, and
P Robins, and
T J Naven, and
D J Pappin, and
J Sgouros, and
T Lindahl
March 1985,
The Journal of biological chemistry,
M Höss, and
P Robins, and
T J Naven, and
D J Pappin, and
J Sgouros, and
T Lindahl
March 1990,
Gene,
M Höss, and
P Robins, and
T J Naven, and
D J Pappin, and
J Sgouros, and
T Lindahl
April 2005,
Research in microbiology,
M Höss, and
P Robins, and
T J Naven, and
D J Pappin, and
J Sgouros, and
T Lindahl
February 1989,
The EMBO journal,
M Höss, and
P Robins, and
T J Naven, and
D J Pappin, and
J Sgouros, and
T Lindahl
May 1984,
The Journal of biological chemistry,
M Höss, and
P Robins, and
T J Naven, and
D J Pappin, and
J Sgouros, and
T Lindahl
April 1983,
Proceedings of the National Academy of Sciences of the United States of America,
