Augmented acetylcholine-induced, Rho-mediated Ca2+ sensitization of bronchial smooth muscle contraction in antigen-induced airway hyperresponsive rats. 1999

Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
Department of Pharmacology, School of Pharmacy, Hoshi University, Tokyo, Japan. chiba@hoshi.ac.jp

Treatment with acetylcholine (ACh) of a beta-escin-permeabilized intrapulmonary bronchial smooth muscle of the rat induced force when the Ca2+ concentration was clamped at 1 microM. The ACh-induced Ca2+ sensitization of myofilaments was significantly greater in antigen-induced airway hyperresponsive rats than in control rats. The ACh-induced Ca2+ sensitization was completely blocked by treatment with Clostridium botulinum C3 exoenzyme, an inactivator of Rho family of proteins. Moreover, the protein level of RhoA in the intrapulmonary bronchi was significantly increased in the airway hyperresponsive rats. Thus, increased airway smooth muscle contractility observed in asthmatics may be related to augmented agonist-induced, Rho-mediated Ca2+ sensitization of myofilaments.

UI MeSH Term Description Entries
D008297 Male Males
D008841 Actin Cytoskeleton Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments. Actin Filaments,Microfilaments,Actin Microfilaments,Actin Cytoskeletons,Actin Filament,Actin Microfilament,Cytoskeleton, Actin,Cytoskeletons, Actin,Filament, Actin,Filaments, Actin,Microfilament,Microfilament, Actin,Microfilaments, Actin
D009119 Muscle Contraction A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments. Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009130 Muscle, Smooth Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed) Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D001980 Bronchi The larger air passages of the lungs arising from the terminal bifurcation of the TRACHEA. They include the largest two primary bronchi which branch out into secondary bronchi, and tertiary bronchi which extend into BRONCHIOLES and PULMONARY ALVEOLI. Primary Bronchi,Primary Bronchus,Secondary Bronchi,Secondary Bronchus,Tertiary Bronchi,Tertiary Bronchus,Bronchi, Primary,Bronchi, Secondary,Bronchi, Tertiary,Bronchus,Bronchus, Primary,Bronchus, Secondary,Bronchus, Tertiary
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D004928 Escin Pentacyclic triterpene saponins, biosynthesized from protoaescigenin and barringtogenol, occurring in the seeds of AESCULUS. It inhibits edema formation and decreases vascular fragility. Aescin,Aescusan,Escina,Eskuzan,Fepalitan,Feparil,Flebostasin,Opino,Reparil,beta-Aescin,beta-Escin,opino-biomo,beta Aescin,beta Escin,opino biomo,opinobiomo
D000109 Acetylcholine A neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system. 2-(Acetyloxy)-N,N,N-trimethylethanaminium,Acetilcolina Cusi,Acetylcholine Bromide,Acetylcholine Chloride,Acetylcholine Fluoride,Acetylcholine Hydroxide,Acetylcholine Iodide,Acetylcholine L-Tartrate,Acetylcholine Perchlorate,Acetylcholine Picrate,Acetylcholine Picrate (1:1),Acetylcholine Sulfate (1:1),Bromoacetylcholine,Chloroacetylcholine,Miochol,Acetylcholine L Tartrate,Bromide, Acetylcholine,Cusi, Acetilcolina,Fluoride, Acetylcholine,Hydroxide, Acetylcholine,Iodide, Acetylcholine,L-Tartrate, Acetylcholine,Perchlorate, Acetylcholine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000941 Antigens Substances that are recognized by the immune system and induce an immune reaction. Antigen

Related Publications

Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
January 1999, Research communications in molecular pathology and pharmacology,
Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
July 2001, British journal of pharmacology,
Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
April 2001, Biochemical pharmacology,
Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
January 2010, Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi,
Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
December 2003, Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi,
Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
October 2004, Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi,
Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
July 2005, Molecular pharmacology,
Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
September 1999, Nihon yakurigaku zasshi. Folia pharmacologica Japonica,
Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
January 2005, Respiratory research,
Y Chiba, and Y Takada, and S Miyamoto, and M MitsuiSaito, and H Karaki, and M Misawa
October 2019, Journal of pharmacological sciences,
Copied contents to your clipboard!