BIOMAT Database Logo BIOMAT Database Logo

Regulation of gelatinases in microglia and astrocyte cell cultures by plant lectins. 1999

G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
Dipartimento di Biochimica e Biologia Molecolare, University of Bari, Italy. m.g.liuzzi@biologia.uniba.it

The effects of 25 recently discovered plant lectins on cell proliferation and enzyme release were compared to those of previously known lectins on rat microglia and astrocyte cell cultures. A dose-dependent proliferation of microglial cells, but not of astrocytes, was induced by seven lectins, whereas five lectins showed dose-dependent cytotoxicity on both microglia and astrocyte cell cultures. The activity of gelatinase B (MMP-9) was strongly increased in microglial cells by the aforementioned seven lectins, by concanavalin A, and by phytohemagglutinin (PHA-E4), whereas gelatinase A (MMP-2) remained at constitutive levels. The five cytotoxic lectins decreased the activity of gelatinase B in microglia and of gelatinase A in astrocytes, in a dose-dependent manner. The lectin wheat germ agglutinin induced a decrease in gelatinase B activity in microglia, but stimulated gelatinase A and B activity in astrocytes. These results indicate that lectins possess neuromodulatory effects that may motivate the study of their effects on central nervous system (CNS) function in vivo. This, in turn, may lead to better insight into whether lectin or lectin-like molecules can interact with glial cells, and whether they have a role in acute toxicity and in multifactorial diseases in which environmental factors may play a role.

UI MeSH Term Description Entries
D008666 Metalloendopeptidases ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism. Metallo-Endoproteinases,Metalloendopeptidase
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001253 Astrocytes A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury. Astroglia,Astroglia Cells,Astroglial Cells,Astrocyte,Astroglia Cell,Astroglial Cell,Astroglias,Cell, Astroglia,Cell, Astroglial
D017364 Collagenases Enzymes that catalyze the degradation of collagen by acting on the peptide bonds. Collagen Peptidase,Collagen-Degrading Enzyme,Collagenase,Collagen Degrading Enzyme,Peptidase, Collagen
D017628 Microglia The third type of glial cell, along with astrocytes and oligodendrocytes (which together form the macroglia). Microglia vary in appearance depending on developmental stage, functional state, and anatomical location; subtype terms include ramified, perivascular, ameboid, resting, and activated. Microglia clearly are capable of phagocytosis and play an important role in a wide spectrum of neuropathologies. They have also been suggested to act in several other roles including in secretion (e.g., of cytokines and neural growth factors), in immunological processing (e.g., antigen presentation), and in central nervous system development and remodeling. Microglial Cell,Cell, Microglial,Microglial Cells,Microglias
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D018093 Gelatinases A class of enzymes that catalyzes the degradation of gelatin by acting on the peptide bonds. EC 3.4.24.-. Gelatinase
D020778 Matrix Metalloproteinase 2 A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain. Gelatinase A,72-kDa Gelatinase,72-kDa Type IV Collagenase,MMP-2 Metalloproteinase,MMP2 Metalloproteinase,Matrix Metalloproteinase-2,72 kDa Gelatinase,72 kDa Type IV Collagenase,Gelatinase, 72-kDa,MMP 2 Metalloproteinase,Metalloproteinase 2, Matrix,Metalloproteinase, MMP-2,Metalloproteinase, MMP2

Related Publications

G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
Opposite regulation of adenylyl cyclase by protein kinase C in astrocyte and microglia cultures.
September 1997, Journal of neurochemistry,
G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
Generation of primary astrocyte cultures devoid of contaminating microglia.
January 2012, Methods in molecular biology (Clifton, N.J.),
G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
Liposomal clodronate selectively eliminates microglia from primary astrocyte cultures.
May 2012, Journal of neuroinflammation,
G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
Derivation of microglia-free astrocyte cultures from neural stem cells.
January 2012, Methods in molecular biology (Clifton, N.J.),
G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
The effect of activated microglia on astrogliosis parameters in astrocyte cultures.
January 2007, Brain research,
G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
Inhibition of myelin-cleaving poteolytic activities by interferon-beta in rat astrocyte cultures. Comparative analysis between gelatinases and calpain-II.
January 2013, PloS one,
G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
Regulation of angiotensinogen by angiotensin II in mouse primary astrocyte cultures.
October 2011, Journal of neurochemistry,
G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
A novel method to establish microglia-free astrocyte cultures: comparison of matrix metalloproteinase expression profiles in pure cultures of astrocytes and microglia.
August 2008, Glia,
G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
Regulation of gelatinase B (MMP-9) in leukocytes by plant lectins.
May 1998, FEBS letters,
G M Liuzzi, and M P Santacroce, and W J Peumans, and E J Van Damme, and B Dubois, and G Opdenakker, and P Riccio
Regulation of trophoblastic gelatinases by proto-oncogenes.
January 2003, Placenta,
Copied contents to your clipboard!