Biomaterial Database

The distribution of sodium, potassium and chloride in the nucleus and cytoplasm of Bufo bufo oocytes measured by electron microprobe analysis. 1978

D A Dick

1. Measurements of cytoplasmic and nuclear Na, K and Cl have been made by electron microprobe analysis on freeze-dried sections of oocytes of Bufo bufo, using standards of bovine plasma albumin and gamma-globulin. Concentrations were obtained per kilogram of dry mass, were converted to concentrations per litre of water content using known figures for water and solid concentration of nucleus and cytoplasm, and were then compared with measurements on cells from the same animal obtained by flame photometry. 2. In fresh oocytes concentrations were (mean +/- S.E. of mean in m-mole/l. H2O) in cytoplasm Na 10.9 +/- 1.95, K 70.2 +/- 3.22, Cl 98.8 +/- 11.0, and in nucleus Na 10.4 +/- 1.79, K 266.4 +/- 22.8, Cl 91.3 +/- 9.0. 3. After treatment with Na-free Ringer (Li substituted for Na) for 5 hr, concentrations were in cytoplasm Na 11.1 +/- 2.44, K 64.4 +/- 5.7, Cl 88.7 +/- 8.8, and in nucleus Na 2.4 +/- 0.73, K 141 +/- 13.9, Cl 75.0 +/- 6.7. Na inexchangeable with Li therefore lay in the cytoplasm but not in the nucleus as previously shown by autoradiography. 4. For K electron microscopic analysis measurements agreed well with those obtained by flame photometry but the former measured only 35% of Na measured by flame photometry. This discrepancy may be due either to technical difficulties with the electron microprobe analysis or to localization of Na in the cytoplasm.

UI	MeSH Term	Description	Entries
D009865	Oocytes	Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).	Ovocytes,Oocyte,Ovocyte
D010063	Ovum	A mature haploid female germ cell extruded from the OVARY at OVULATION.	Egg,Egg, Unfertilized,Ova,Eggs, Unfertilized,Unfertilized Egg,Unfertilized Eggs
D010783	Photometry	Measurement of the various properties of light.	Photometries
D011188	Potassium	An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
D002023	Bufo bufo	A species of the true toads, Bufonidae, widely distributed in the United States and Europe.	Toad, Common,Common Toad,Common Toads,Toads, Common
D002467	Cell Nucleus	Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D002712	Chlorides	Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.	Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D003593	Cytoplasm	The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)	Protoplasm,Cytoplasms,Protoplasms
D004577	Electron Probe Microanalysis	Identification and measurement of ELEMENTS and their location based on the fact that X-RAYS emitted by an element excited by an electron beam have a wavelength characteristic of that element and an intensity related to its concentration. It is performed with an electron microscope fitted with an x-ray spectrometer, in scanning or transmission mode.	Microscopy, Electron, X-Ray Microanalysis,Spectrometry, X-Ray Emission, Electron Microscopic,Spectrometry, X-Ray Emission, Electron Probe,X-Ray Emission Spectrometry, Electron Microscopic,X-Ray Emission Spectrometry, Electron Probe,X-Ray Microanalysis, Electron Microscopic,X-Ray Microanalysis, Electron Probe,Microanalysis, Electron Probe,Spectrometry, X Ray Emission, Electron Microscopic,Spectrometry, X Ray Emission, Electron Probe,X Ray Emission Spectrometry, Electron Microscopic,X Ray Emission Spectrometry, Electron Probe,X-Ray Microanalysis,Electron Probe Microanalyses,Microanalyses, Electron Probe,Microanalysis, X-Ray,Probe Microanalyses, Electron,Probe Microanalysis, Electron,X Ray Microanalysis,X Ray Microanalysis, Electron Microscopic,X Ray Microanalysis, Electron Probe
D005260	Female		Females