BIOMAT Database Logo BIOMAT Database Logo

Glial cell line-derived neurotrophic factor induces barrier function of endothelial cells forming the blood-brain barrier. 1999

Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
Department of Pathology, Department of Ophthalmology, Department of Neurosurgery, Sapporo Medical University School of Medicine, South-1, West-17, Chuo-ku, 060, Japan.

Since a deep involvement of astrocytes, a kind of glial cells, in differentiation of the blood-brain barrier (BBB) has been suggested, we examined the relation of glial cell line-derived neurotrophic factor (GDNF) to the BBB. First, immunohistochemical examination of the cerebral cortex of rats revealed that glial cell line-derived neurotrophic factor receptor (GFRalpha1) was preferentially expressed on the cell membranes of capillary endothelial cells. Second, to elucidate the effects of GDNF on the BBB, capillary endothelial cells isolated from the porcine cerebral cortex were cultured and then changes in tight junction function of the endothelial cells were examined after addition of GDNF, in terms of transendothelial electrical resistance (TER) and permeability. GDNF at concentrations of 0.1 and 1 ng/ml significantly activated the barrier function of the endothelial cells in the presence of cAMP. Since GDNF is secreted from astrocytes sheathing capillary endothelial cells in the brain cortex, our results strongly suggest that GDNF enhances the barrier function of tight junctions of the BBB on the one hand, and also supports the survival of neurons on the other hand.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D009414 Nerve Growth Factors Factors which enhance the growth potentialities of sensory and sympathetic nerve cells. Neurite Outgrowth Factor,Neurite Outgrowth Factors,Neuronal Growth-Associated Protein,Neuronotrophic Factor,Neurotrophic Factor,Neurotrophic Factors,Neurotrophin,Neurotrophins,Growth-Associated Proteins, Neuronal,Neuronal Growth-Associated Proteins,Neuronotrophic Factors,Neurotrophic Protein,Neurotrophic Proteins,Proteins, Neuronal Growth-Associated,Factor, Neurite Outgrowth,Factor, Neuronotrophic,Factor, Neurotrophic,Factors, Nerve Growth,Factors, Neurite Outgrowth,Factors, Neuronotrophic,Factors, Neurotrophic,Growth Associated Proteins, Neuronal,Growth-Associated Protein, Neuronal,Neuronal Growth Associated Protein,Neuronal Growth Associated Proteins,Outgrowth Factor, Neurite,Outgrowth Factors, Neurite,Protein, Neuronal Growth-Associated
D009419 Nerve Tissue Proteins Proteins, Nerve Tissue,Tissue Proteins, Nerve
D011518 Proto-Oncogene Proteins Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity. Cellular Proto-Oncogene Proteins,c-onc Proteins,Proto Oncogene Proteins, Cellular,Proto-Oncogene Products, Cellular,Cellular Proto Oncogene Proteins,Cellular Proto-Oncogene Products,Proto Oncogene Products, Cellular,Proto Oncogene Proteins,Proto-Oncogene Proteins, Cellular,c onc Proteins
D001812 Blood-Brain Barrier Specialized non-fenestrated tightly-joined ENDOTHELIAL CELLS with TIGHT JUNCTIONS that form a transport barrier for certain substances between the cerebral capillaries and the BRAIN tissue. Brain-Blood Barrier,Hemato-Encephalic Barrier,Barrier, Blood-Brain,Barrier, Brain-Blood,Barrier, Hemato-Encephalic,Barriers, Blood-Brain,Barriers, Brain-Blood,Barriers, Hemato-Encephalic,Blood Brain Barrier,Blood-Brain Barriers,Brain Blood Barrier,Brain-Blood Barriers,Hemato Encephalic Barrier,Hemato-Encephalic Barriers
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002463 Cell Membrane Permeability A quality of cell membranes which permits the passage of solvents and solutes into and out of cells. Permeability, Cell Membrane
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002540 Cerebral Cortex The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulci. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions. Allocortex,Archipallium,Cortex Cerebri,Cortical Plate,Paleocortex,Periallocortex,Allocortices,Archipalliums,Cerebral Cortices,Cortex Cerebrus,Cortex, Cerebral,Cortical Plates,Paleocortices,Periallocortices,Plate, Cortical
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response

Related Publications

Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
Glial cell line-derived neurotrophic factor induces neuronal sprouting in human brain.
July 2005, Nature medicine,
Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
Amphotericin B induces glial cell line-derived neurotrophic factor in the rat brain.
October 2014, The Journal of veterinary medical science,
Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
Citrus Auraptene Induces Glial Cell Line-Derived Neurotrophic Factor in C6 Cells.
December 2019, International journal of molecular sciences,
Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
Pericyte-derived glial cell line-derived neurotrophic factor increase the expression of claudin-5 in the blood-brain barrier and the blood-nerve barrier.
February 2012, Neurochemical research,
Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
In vivo delivery of glial cell-derived neurotrophic factor across the blood-brain barrier by gene transfer into brain capillary endothelial cells.
August 2003, Human gene therapy,
Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
Glial cell line-derived neurotrophic factor induces Ret-mediated lamellipodia formation.
January 1997, The Journal of biological chemistry,
Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
S100B protein, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor in human milk.
January 2011, PloS one,
Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
Enhanced delivery of [125I]glial cell line-derived neurotrophic factor to the rat CNS following osmotic blood-brain barrier modification.
December 1996, Neuroscience letters,
Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
Barrier function of microvessels and roles of glial cell line-derived neurotrophic factor in the rat testis.
September 2002, Medical electron microscopy : official journal of the Clinical Electron Microscopy Society of Japan,
Y Igarashi, and H Utsumi, and H Chiba, and Y Yamada-Sasamori, and H Tobioka, and Y Kamimura, and K Furuuchi, and Y Kokai, and T Nakagawa, and M Mori, and N Sawada
Human glial cell-line-derived neurotrophic factor: a structure-function analysis.
February 2000, Biochemical and biophysical research communications,
Copied contents to your clipboard!