Biomaterial Database

Two distinct HCO(-)(3)-dependent H(+) efflux pathways in human vascular endothelial cells. 1999

B Sun, and R D Vaughan-Jones, and J Kambayashi

University Laboratory of Physiology, Oxford OX1 3PT, United Kingdom. bings@mrl.oapi.com

Intracellular pH (pH(i)) regulation in human umbilical vein endothelial cells (HUVEC) was investigated. The pH(i) was recorded using seminaphthorhodafluor-1 (SNARF-1). Cells were intracellularly acid loaded with NH(4)Cl prepulse. In HEPES-buffered Tyrode (nominally HCO(-)(3) free), pH(i) recovery from acid load was inhibited by 1.5 mM amiloride or Na(+)-free solution. Additionally, in HCO(-)(3)-buffered Tyrode, a HCO(-)(3)-dependent pH(i) recovery from acidosis was evident in the presence of 1.5 mM amiloride, which mediated complete recovery of pH(i) (7.26). In Na(+)-free solution, the HCO(-)(3)-dependent acid extruder mediated pH(i) recovery after an acid load but only back to 7.09. These results suggest that there are two HCO(-)(3)-dependent acid extruders in the HUVEC. One is Na(+) dependent, and the other is Na(+) independent. The former was further shown to be completely inhibited by 0.5 mM DIDS, whereas the latter was only inhibited by 24.6%. In Cl(-)-free solution, both of the HCO(-)(3)-dependent pathways were inhibited. In conclusion, one HCO(-)(3)-dependent acid extruder in the HUVEC resembles the Na(+)-dependent Cl(-)/HCO(-)(3) exchange found in other tissues, and the other is Cl(-) dependent but Na(+) independent.

UI	MeSH Term	Description	Entries
D007552	Isotonic Solutions	Solutions having the same osmotic pressure as blood serum, or another solution with which they are compared. (From Grant & Hackh's Chemical Dictionary, 5th ed & Dorland, 28th ed)	Solutions, Isotonic
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004730	Endothelium, Vascular	Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.	Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D001639	Bicarbonates	Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity.	Bicarbonate,Bicarbonate Ions,Hydrogen Carbonates,Bicarbonate Ion,Carbonic Acid Ions,Hydrogen Carbonate,Carbonate, Hydrogen,Carbonates, Hydrogen,Ion, Bicarbonate,Ions, Bicarbonate,Ions, Carbonic Acid
D017923	Sodium-Hydrogen Exchangers	A family of plasma membrane exchange glycoprotein antiporters that transport sodium ions and protons across lipid bilayers. They have critical functions in intracellular pH regulation, cell volume regulation, and cellular response to many different hormones and mitogens.	Na(+)-H(+)-Antiporter,Na(+)-H(+)-Exchanger,Sodium-Hydrogen Antiporter,Na(+)-H(+)-Antiporters,Na(+)-H(+)-Exchangers,SLC9 Na(+)-H(+) Exchangers,SLC9 Protein Family,SLC9 Proteins,SLC9-NHE Protein Family,Sodium-Hydrogen Antiporters,Sodium-Hydrogen Exchanger,Sodium-Proton Antiporter,Sodium-Proton Antiporters,Solute Carrier 9 Protein Family,Solute Carrier 9 Proteins,Antiporter, Sodium-Hydrogen,Antiporter, Sodium-Proton,Antiporters, Sodium-Hydrogen,Antiporters, Sodium-Proton,Exchanger, Sodium-Hydrogen,Exchangers, Sodium-Hydrogen,Protein Family, SLC9,Protein Family, SLC9-NHE,SLC9 NHE Protein Family,Sodium Hydrogen Antiporter,Sodium Hydrogen Antiporters,Sodium Hydrogen Exchanger,Sodium Hydrogen Exchangers,Sodium Proton Antiporter,Sodium Proton Antiporters