Biomaterial Database

Activity of enzymes catalyzing formation of beta-L-fucosyl phosphate and GDP-beta-L-fucose in amphibian tissues and their application in chemo-enzymic synthesis of GDP-beta-L-fucose. 1999

T N Druzhinina, and N S Utkina, and K Chan, and G Strecker, and V N Shibaev

Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia.

Amphibian oviduct and liver were shown to contain enzymes that catalyze the formation of GDP-beta-L-fucose from GDP-alpha-D-mannose or L-fucose. The conversion of L-fucose into beta-L-fucopyranosyl phosphate was achieved on a preparative scale using high-activity fucokinase in toad liver extracts. For chemo-enzymic preparation of GDP-beta-L-fucose, a convenient modification for pyrophosphate synthesis through phosphomorpholidate which does not require anhydrous conditions is suggested.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D010057	Oviducts	Ducts that serve exclusively for the passage of eggs from the ovaries to the exterior of the body. In non-mammals, they are termed oviducts. In mammals, they are highly specialized and known as FALLOPIAN TUBES.	Oviduct
D010999	Pleurodeles	A genus of aquatic newts belonging to the family Salamandridae and sometimes referred to as "spiny" tritons. There are two species P. waltlii and P. poireti. P. waltlii is commonly used in the laboratory. Since this genus adapts to aquarium living, it is easy to maintain in laboratories.	Pleurodele
D002023	Bufo bufo	A species of the true toads, Bufonidae, widely distributed in the United States and Europe.	Toad, Common,Common Toad,Common Toads,Toads, Common
D002384	Catalysis	The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.	Catalyses
D004798	Enzymes	Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.	Biocatalyst,Enzyme,Biocatalysts
D005260	Female		Females
D005643	Fucose	A six-member ring deoxysugar with the chemical formula C6H12O5. It lacks a hydroxyl group on the carbon at position 6 of the molecule.	Deoxygalactose,alpha-Fucose,alpha Fucose
D006154	Guanosine Diphosphate Fucose	A nucleoside diphosphate sugar formed from GDPmannose, which provides fucose for lipopolysaccharides of bacterial cell walls, and for blood group substances and other glycoproteins.	GDP Fucose,Guanosine Diphosphofucose,Diphosphate Fucose, Guanosine,Diphosphofucose, Guanosine,Fucose, GDP,Fucose, Guanosine Diphosphate
D006600	Hexosephosphates