Sequence analysis, characterization and CO-specific transcription of the cox gene cluster on the megaplasmid pHCG3 of Oligotropha carboxidovorans. 1999

B Santiago, and U Schübel, and C Egelseer, and O Meyer
Lehrstuhl für Mikrobiologie, Universität Bayreuth, Universitätsstrasse 30, 95440, Bayreuth, Germany.

Sequence, transcriptional, mutational and physiological analyses indicate that the carbon monoxide (CO) dehydrogenase of Oligotropha carboxidovorans is an integral and unique part of an elaborate CO oxidizing system. It is encoded by the 14.5kb gene cluster coxBCMSLDEFGHIK residing on the 128kb megaplasmid pHCG3. The CO dehydrogenase structural genes coxMSL are flanked by nine accessory genes arranged as the cox gene cluster. The cox genes are specifically and coordinately transcribed under chemolithoautotrophic conditions in the presence of CO as carbon and energy source. With the exception of CoxB and CoxK, all deduced products of the cox genes of O. carboxidovorans have counterparts in so far uncharacterized gene clusters of Pseudomonas thermocarboxydovorans, Hydrogenophaga pseudoflava, Bradyrhizobium japonicum, and Mycobacterium tuberculosis. Transposon mutagenesis suggests a function of CoxH and CoxI in the interaction of CO dehydrogenase with the cytoplasmic membrane. The specific functions of the other accessory Cox proteins are difficult to envisage right now, as the polypeptides do not show significant homologies with functionally characterized proteins in the databases. In addition to the clustered cox genes, mutational analyses have identified the genes lon, cycH and orfX which reside on the plasmid pHCG3. The Lon protease, the CycH protein and the unknown orfX gene product have essential functions in the utilization of CO.

UI MeSH Term Description Entries
D008957 Models, Genetic Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment. Genetic Models,Genetic Model,Model, Genetic
D009097 Multienzyme Complexes Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES. Complexes, Multienzyme
D010455 Peptides Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS. Peptide,Polypeptide,Polypeptides
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011549 Pseudomonas A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants. Chryseomonas,Pseudomona,Flavimonas
D002248 Carbon Monoxide Carbon monoxide (CO). A poisonous colorless, odorless, tasteless gas. It combines with hemoglobin to form carboxyhemoglobin, which has no oxygen carrying capacity. The resultant oxygen deprivation causes headache, dizziness, decreased pulse and respiratory rates, unconsciousness, and death. (From Merck Index, 11th ed) Monoxide, Carbon
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D005810 Multigene Family A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed) Gene Clusters,Genes, Reiterated,Cluster, Gene,Clusters, Gene,Families, Multigene,Family, Multigene,Gene Cluster,Gene, Reiterated,Multigene Families,Reiterated Gene,Reiterated Genes
D005838 Genotype The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS. Genogroup,Genogroups,Genotypes

Related Publications

B Santiago, and U Schübel, and C Egelseer, and O Meyer
March 2015, Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry,
B Santiago, and U Schübel, and C Egelseer, and O Meyer
August 2008, Journal of bacteriology,
B Santiago, and U Schübel, and C Egelseer, and O Meyer
January 2020, Biochimica et biophysica acta. Bioenergetics,
B Santiago, and U Schübel, and C Egelseer, and O Meyer
April 2010, The Journal of biological chemistry,
B Santiago, and U Schübel, and C Egelseer, and O Meyer
May 2018, Biochemistry,
B Santiago, and U Schübel, and C Egelseer, and O Meyer
September 2010, BMC genomics,
B Santiago, and U Schübel, and C Egelseer, and O Meyer
February 2017, Carbohydrate research,
B Santiago, and U Schübel, and C Egelseer, and O Meyer
December 2014, Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry,
Copied contents to your clipboard!