BIOMAT Database Logo BIOMAT Database Logo

Immunoglobulin M antibody test to detect genogroup II Norwalk-like virus infection. 1999

J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.

Sera obtained from adult volunteers inoculated with genogroup II Norwalk-like viruses (NLVs), Hawaii virus, and Snow Mountain virus and from patients involved in outbreaks of gastroenteritis were tested for genogroup II NLV Mexico virus-specific immunoglobulin M (IgM) by use of a monoclonal antibody, recombinant Mexico virus antigen (rMXV)-based IgM capture enzyme-linked immunosorbent assay (ELISA). Sera from genogroup I Norwalk virus (NV)-inoculated volunteers and from patients involved in a genogroup I NLV outbreak were also tested. In sera from those infected with genogroup I NV or NLVs in volunteer and outbreak studies, only 3 of 25 were rMXV IgM positive; in contrast, 24 of 25 were IgM positive for recombinant NV (rNV). In sera from those infected with genogroup II NLVs in volunteer and outbreak studies, 28 of 47 were rMXV IgM positive and none were IgM positive for rNV, showing the specificity of each IgM test for its respective genogroup. In an outbreak of gastroenteritis not characterized as being of viral etiology but suspected to be due to NV, 7 of 13 persons had IgM responses to rMXV, whereas none had IgM responses to rNV, thus establishing the diagnosis as genogroup II NLV infection. The rMXV-based IgM capture ELISA developed is specific for the diagnosis of genogroup II NLV infections.

UI MeSH Term Description Entries
D007075 Immunoglobulin M A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin. Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D007223 Infant A child between 1 and 23 months of age. Infants
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009663 Norwalk virus The type species in the genus NOROVIRUS, first isolated in 1968 from the stools of school children in Norwalk, Ohio, who were suffering from GASTROENTERITIS. The virions are non-enveloped spherical particles containing a single protein. Multiple strains are named after the places where outbreaks have occurred. Norwalk Agent
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002675 Child, Preschool A child between the ages of 2 and 5. Children, Preschool,Preschool Child,Preschool Children
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults

Related Publications

J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
[Outbreak of foodborne disease by Norwalk-like viruses genogroup II].
June 2002, Medicina clinica,
J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
Immunoglobulin M responses to the Norwalk virus of gastroenteritis.
August 1982, Infection and immunity,
J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
Serum immunoglobulin A response to Norwalk virus infection.
June 1989, Journal of clinical microbiology,
J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
Experimental human infection with Norwalk virus elicits a surrogate neutralizing antibody response with cross-genogroup activity.
February 2015, Clinical and vaccine immunology : CVI,
J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
Improved Epstein-Barr virus immunoglobulin M antibody test.
February 1975, Journal of clinical microbiology,
J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
Antigenic diversity of Norwalk-like viruses: expression of the capsid protein of a genogroup I virus, distantly related to Norwalk virus.
January 2000, Archives of virology,
J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
Expression of recombinant capsid proteins of chitta virus, a genogroup II Norwalk virus, and development of an ELISA to detect the viral antigen.
January 2000, Microbiology and immunology,
J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
Molecular cloning, expression, and antigenicity of Seto virus belonging to genogroup I Norwalk-like viruses.
September 2000, Journal of clinical microbiology,
J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
The seroepidemiology of genogroup 1 and genogroup 2 Norwalk-like viruses in Italy.
May 1999, Journal of medical virology,
J P Brinker, and N R Blacklow, and X Jiang, and M K Estes, and C L Moe, and J E Herrmann
Identification of an epitope common to genogroup 1 "norwalk-like viruses".
April 2000, Journal of clinical microbiology,
Copied contents to your clipboard!