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In the present study we examined the effect of sulfonylurea on the expression of the glucose transporter GLUT2 and the glucose phosphorylating enzyme Glucokinase (GK) in betaTC6-F7 cells; furthermore, we studied the modifications induced by sulfonylurea on glucose-responsiveness and -sensitivity. Results demonstrate that sulfonylurea increases GLUT2 and GK mRNA expression after 24 h in a dose-dependent manner. On the contrary, after 48 and 72 h a time-dependent reduction of both GLUT2 and GK mRNA occurs. GLUT2 and GK protein expression follow the same modifications. Therefore, GLUT2 and GK are coordinately regulated by sulfonylurea, probably by a common mechanism. Glucose-induced insulin release is increased by sulfonylurea as well as glucose sensitivity. Our study suggests that short-term effect of sulfonylurea increases while long-term effect reduces the expression of glucose sensing elements. The long-term inhibitory effect on glucose sensing elements would explain the reduced insulin secretion occurring after chronic sulfonylurea treatment.
O Porzio, and
L N Marlier, and
M Federici, and
M L Hribal, and
R Magnaterra, and
D Lauro, and
A Fusco, and
G Sesti, and
P Borboni
September 2016,
Biochemical and biophysical research communications,
O Porzio, and
L N Marlier, and
M Federici, and
M L Hribal, and
R Magnaterra, and
D Lauro, and
A Fusco, and
G Sesti, and
P Borboni
December 2004,
Journal of immunology (Baltimore, Md. : 1950),
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L N Marlier, and
M Federici, and
M L Hribal, and
R Magnaterra, and
D Lauro, and
A Fusco, and
G Sesti, and
P Borboni
June 2015,
Molecular and cellular biology,
O Porzio, and
L N Marlier, and
M Federici, and
M L Hribal, and
R Magnaterra, and
D Lauro, and
A Fusco, and
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P Borboni
June 2006,
Cell biology international,
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A Fusco, and
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P Borboni
June 1997,
Diabetes,
O Porzio, and
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M L Hribal, and
R Magnaterra, and
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A Fusco, and
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P Borboni
February 1994,
Biochemical Society transactions,
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M L Hribal, and
R Magnaterra, and
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G Sesti, and
P Borboni
June 2020,
Genes,
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L N Marlier, and
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R Magnaterra, and
D Lauro, and
A Fusco, and
G Sesti, and
P Borboni
September 2019,
International journal of molecular sciences,
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July 2008,
The Journal of comparative neurology,
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A Fusco, and
G Sesti, and
P Borboni
February 2011,
Journal of orthopaedic research : official publication of the Orthopaedic Research Society,
