BIOMAT Database Logo BIOMAT Database Logo

Effect of Apis mellifera propolis from two Brazilian regions on caries development in desalivated rats. 1999

H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
Department of Physiological Sciences, Faculty of Dentistry of Piracicaba, State University of Campinas, Piracicaba, Brazil.

The purpose of the present study was to evaluate the effect of Apis mellifera propolis collected from two regions of Brazil on caries development in desalivated rats. Ethanolic extracts of propolis (EEP) were prepared from crude propolis samples collected in Minas Gerais state (MG), southeastern Brazil, and Rio Grande do Sul state (RS), southern Brazil. The flavonoid composition of EEP was analyzed by high-performance thin-layer chromatography (HPTLC) and reversed-phase high-performance liquid chromatography (HPLC). For the animal study, 30 specific pathogen-free Wistar rats were infected with Streptococcus sobrinus 6715 and surgically desalivated. The rats were randomly divided into three groups which were treated with 80% ethanol (control), EEP from MG and EEP from RS. The animals were placed in a König-Höfer programmed feeder and received 17 meals of diet 2000 daily at hourly intervals. The solutions were applied on the rat molars (25 microl on molars of each quadrant) twice a day, by using graduate syringes. After 3 weeks, the animals were killed by CO(2) asphyxiation. For microbial assessment, the left jaw was removed and sonicated in 154 mM NaCl solution. Dental caries was evaluated according to Larson's modification of Keyes' system. The HPTLC patterns and HPLC profiles demonstrated that both quality and quantity of flavonoid aglycones of EEP from MG were different compared to EEP from RS. In general, it is apparent that EEP from RS contained the highest concentrations of pinocembrin, chrysin, acacetin and galangin. The group of animals treated with EEP from RS showed the lowest smooth-surface and sulcal caries scores as well as less caries severity in smooth-surface and sulcal lesions, and these data were statistically different when compared with the control group. The group treated with EEP from MG only demonstrated a significant difference in the severity of sulcal lesions when compared to the control group. The percentage of S. sobrinus was lower in the groups treated with EEP, but did not differ statistically from the control group. The results showed that the cariostatic effect of propolis depends on its composition, and consequently the region of collection of propolis samples.

UI MeSH Term Description Entries
D011429 Propolis A resinous substance obtained from beehives that is used traditionally as an antimicrobial. It is a heterogeneous mixture of many substances. Bee Bread,Bee Glue,Bread, Bee,Glue, Bee
D011897 Random Allocation A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects. Randomization,Allocation, Random
D001938 Brazil A country located on the eastern coast of South America, located between Colombia and Peru, that borders the Atlantic Ocean. It is bordered on the north by Venezuela, Guyana, Suriname, and French Guiana, on the south by Uruguay, and on the west by Argentina. The capital is Brasilia.
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002855 Chromatography, Thin Layer Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D003731 Dental Caries Localized destruction of the tooth surface initiated by decalcification of the enamel followed by enzymatic lysis of organic structures and leading to cavity formation. If left unchecked, the cavity may penetrate the enamel and dentin and reach the pulp. Caries, Dental,Carious Lesions,Dental Cavities,Dental Cavity,Dental Decay,Dental White Spots,Carious Dentin,Decay, Dental,Dental White Spot,White Spot, Dental,White Spots, Dental,Carious Dentins,Carious Lesion,Cavities, Dental,Cavity, Dental,Dentin, Carious,Dentins, Carious,Lesion, Carious,Lesions, Carious,Spot, Dental White,Spots, Dental White
D005419 Flavonoids A group of phenyl benzopyrans named for having structures like FLAVONES. 2-Phenyl-Benzopyran,2-Phenyl-Chromene,Bioflavonoid,Bioflavonoids,Flavonoid,2-Phenyl-Benzopyrans,2-Phenyl-Chromenes,2 Phenyl Benzopyran,2 Phenyl Benzopyrans,2 Phenyl Chromene,2 Phenyl Chromenes
D000704 Analysis of Variance A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable. ANOVA,Analysis, Variance,Variance Analysis,Analyses, Variance,Variance Analyses
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001516 Bees Insect members of the superfamily Apoidea, found almost everywhere, particularly on flowers. About 3500 species occur in North America. They differ from most WASPS in that their young are fed honey and pollen rather than animal food. Apidae,Apis,Apis mellifera,Apis mellifica,European Honey Bee,Honey Bee Drone,Bee,Bee, European Honey,Drone, Honey Bee,European Honey Bees,Honey Bee Drones,Honey Bee, European

Related Publications

H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
Antitrypanosomal activity of Brazilian propolis from Apis mellifera.
May 2004, Chemical & pharmaceutical bulletin,
H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
Effect of copper co-crystallized with sugar on caries development in desalivated rats.
January 1996, Caries research,
H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
Pilocarpine alters caries development in partially-desalivated rats.
March 1994, Journal of dental research,
H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
[Effect of Mexican propolis extracts from Apis mellifera on Candida albicans in vitro growth].
March 2008, Revista iberoamericana de micologia,
H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
Food-grade delivery systems of Brazilian propolis from Apis mellifera: From chemical composition to bioactivities in vivo.
January 2024, Food chemistry,
H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
Radionuclides in honeybee propolis (Apis mellifera L.).
April 2006, Bulletin of environmental contamination and toxicology,
H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
The effect of sex hormones on dental caries in desalivated rats.
October 1957, Journal of dental research,
H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
Antibacterial activity of a cardanol from Thai Apis mellifera propolis.
January 2014, International journal of medical sciences,
H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
Influence of fluoride co-cystallized with sugar on caries development in desalivated rats.
April 1997, Archives of oral biology,
H Koo, and P L Rosalen, and J A Cury, and Y K Park, and M Ikegaki, and A Sattler
The effects of cheese snacks on caries in desalivated rats.
June 1987, Journal of dental research,
Copied contents to your clipboard!