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Application of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) to identification of flatfish species. 1999
A Céspedes, and
T García, and
E Carrera, and
I González, and
A Fernández, and
P E Hernández, and
R Martín
Universidad Complutense, Facultad de Veterinaria, Departmento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Madrid, Spain.
A method of DNA analysis based on polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).
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