BIOMAT Database Logo BIOMAT Database Logo

A beta-1,4-endoglucanase-encoding gene from Cellulomonas pachnodae. 1999

A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
Department of Microbiology and Evolutionary Biology, Faculty of Science, University of Nijmegen, The Netherlands.

A gene library of Cellulomonas pachnodae was constructed in Escherichia coli and was screened for endoglucanase activity. Five endoglucanase-positive clones were isolated that carried identical DNA fragments. The gene, designated cel6A, encoding an endoglucanase enzyme, belongs to the glycosyl hydrolase family 6 (cellulase family B). The recombinant Cel6A had a molecular mass of 53 kDa, a pH optimum of 5.5, and a temperature optimum of 50-55 degrees C. The recombinant endoglucanase Cel6A bound to crystalline cellulose and beech litter. Based on amino acid sequence similarity, a clear cellulose-binding domain was not distinguished. However, the regions in the Cel6A amino acid sequence at the positions 262-319 and 448-473, which did not show similarity to any of the known family-6 glycosyl hydrolases, may be involved in substrate binding.

UI MeSH Term Description Entries
D007313 Insecta Members of the phylum ARTHROPODA composed or organisms characterized by division into three parts: head, thorax, and abdomen. They are the dominant group of animals on earth with several hundred thousand different kinds. Three orders, HEMIPTERA; DIPTERA; and SIPHONAPTERA; are of medical interest in that they cause disease in humans and animals. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p1). Insects,Insect
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002480 Cellulase An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans. Endo-1,4-beta-Glucanase,Cellulysin,Endoglucanase,Endoglucanase A,Endoglucanase C,Endoglucanase E,Endoglucanase IV,Endoglucanase Y,beta-1,4-Glucan-4-Glucanohydrolase,Endo 1,4 beta Glucanase,beta 1,4 Glucan 4 Glucanohydrolase
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004064 Digestive System A group of organs stretching from the MOUTH to the ANUS, serving to breakdown foods, assimilate nutrients, and eliminate waste. In humans, the digestive system includes the GASTROINTESTINAL TRACT and the accessory glands (LIVER; BILIARY TRACT; PANCREAS). Ailmentary System,Alimentary System
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein

Related Publications

A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase.
April 1993, Journal of bacteriology,
A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
Expression and characterization of the celcflB gene from Cellulomonas flavigena encoding an endo-beta-1,4-glucanase.
November 2003, Current microbiology,
A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
Purification, characterization and modular organization of a cellulose-binding protein, CBP105, a processive beta-1,4-endoglucanase from Cellulomonas flavigena.
April 2008, Biotechnology letters,
A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
Cloning and expression of a beta-1,4-endoglucanase gene from Cellulomonas sp. CelB7 in Escherichia coli; purification and characterization of the recombinant enzyme.
December 1996, FEMS microbiology letters,
A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
Multigene families of Cellulomonas flavigena encoding endo-beta-1,4-glucanases (CM-cellulases).
December 1988, Gene,
A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
Characterization of a thermostable endoglucanase from Cellulomonas fimi ATCC484.
February 2018, Biochemistry and cell biology = Biochimie et biologie cellulaire,
A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
Genes from Cellvibrio mixtus encoding beta-1,3 endoglucanase.
October 1990, Applied and environmental microbiology,
A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
Cloning and sequencing of beta-1,4-endoglucanase gene (celA) from Pseudomonas sp. YD-15.
December 1999, Letters in applied microbiology,
A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
A gene encoding endo-1,4-beta-glucanase from Bacillus sp. 22-28.
February 1997, Bioscience, biotechnology, and biochemistry,
A E Cazemier, and J C Verdoes, and H J Op den Camp, and J H Hackstein, and A J van Ooyen
The gene encoding the beta-1,4-endoglucanase (CelA) from Myxococcus xanthus: evidence for independent acquisition by horizontal transfer of binding and catalytic domains from actinomycetes.
May 1995, Gene,
Copied contents to your clipboard!